癌变·畸变·突变 ›› 2008, Vol. 20 ›› Issue (4): 254-257.doi: 10.3969/j.issn.1004-616x.2008.04.002

• 论著 • 上一篇    下一篇

食管鳞状细胞癌中MGMT和p16基因启动子甲基化关系分析

杨　霞1/ 杨胜利1/ 吕全军2/ 闫爱华1   

  1. 1.郑州大学第一附属医院食管癌研究室,河南　郑州　450052； 2.郑州大学公共卫生学院营养与食品教研室,河南　郑州　450052
  • 收稿日期:2008-04-10 修回日期:2008-05-04 出版日期:2008-07-30 发布日期:2008-07-30

Analysis on the Methylation of the Promoter of MGMT Gene and p16 Gene in Esophageal

YANG Xia1,YANG Sheng_li1,, LU Quan_jun2， YAN Ai_hua1   

  1. 1.Department of Esophageal Cancer Research, The First Affiliated Hospital, Zhengzhou University，Zhengzhou 450052, Henan; 2. Department of Nutrition and Food Hygiene,College of Public Health, Zhengzhou University, Zhengzhou 450052, Henan,China
  • Received:2008-04-10 Revised:2008-05-04 Online:2008-07-30 Published:2008-07-30

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摘要： 背景与目的： 探讨食管鳞状细胞癌中O6_甲基鸟嘌呤_DNA甲基转移酶(O6_methylguanine DNA methyltransferase，MGMT)基因和p16基因启动子的甲基化情况，了解这两个基因启动子甲基化与食管癌发生的关系。 材料与方法： 采用甲基化特异性PCR(MSP)方法分别对44例食管鳞状细胞癌组织进行MGMT基因和p16基因的甲基化检测，并对其甲基化频率分别进行差异性检验和关联性分析。 结果： 在44例食管鳞癌组织中，有18例(40.9％)出现MGMT基因启动子甲基化，23例(52.3％)出现p16基因启动子甲基化，7例(14.9％)两基因均出现甲基化，10例(22.7％)两基因均未出现甲基化。MGMT和p16基因启动子甲基化率差异无统计学意义(P>0.05)。MGMT和p16基因启动子甲基化无明显相关关系(P>0.05)。 结论： MGMT和p16基因启动子甲基化均可能与食管鳞状细胞癌发生、发展过程密切相关，但二者是相互独立进行的。

关键词: 食管鳞状细胞癌, 高甲基化, 表观遗传学, MGMT基因, p16基因

Abstract: BACKGROUND AND AIM: To investigate the promoter methylation state of MGMT (O6_methylguanine DNA methyltransferase) gene and p16 gene in esophageal squamous cell carcinoma, and analyze the methylation frequencies of the two genes, thereby understanding the relationship between methylation of the promoters of MGMT gene and p16 gene in esophageal squamous cell carcinoma. MATERIALS AND METHODS: Methylation of the promoters of MGMT gene and p16 gene was evaluated by methylation_specific PCR in 44 esophageal carcinoma tissues, and carry out differential test and association analysis of the methylation frequencies of the two genes using χ2 test. RESULTS: Among the 44 esophageal squamous cell carcinomas, MGMT gene methylation was found in 18 cases and p16 gene methylation in 23 cases. Both MGMT and p16 gene methylation were present in 7 cases. Neither MGMT nor p16 gene methylation was detected in 10 cases. The difference of methylation frequencies of the two genes was insignificant(P>0.05). Differential test and association analysis of the methylation frequency of two genes was also insignificant. CONCLUSION: Methylation of the promoters of MGMT gene and p16 gene may both be closely associated with the initiation and progression of esophageal squamous cell carcinoma, but the methylations of the two genes took place independently.

Key words: esophageal squamous cell carcinoma, hypermethylation, epigenetics, MGMT gene, p16 gene

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