热休克蛋白HDJ1 影响肺腺癌细胞侵袭和迁移能力的研究

doi:10.3969/j.issn.1004-616x.2017.05.007

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (5): 358-363.doi: 10.3969/j.issn.1004-616x.2017.05.007

热休克蛋白HDJ1 影响肺腺癌细胞侵袭和迁移能力的研究

李雨轩, 李凯, 张彤彤, 郭元彪, 殷俊, 包勇

成都市第三人民医院呼吸内科, 四川成都 610031

收稿日期:2016-11-14 修回日期:2017-06-02 出版日期:2017-09-30 发布日期:2017-09-30
通讯作者: 包勇,E-mail:byong65@163.com E-mail:byong65@163.com
作者简介:李雨轩,E-mail:529905730@qq.com。
基金资助:
四川省应用基础研究计划项目（2014JY0136）；四川省科技厅基金（2014SZ0055）

Heat shock protein HDJ1 on invasion and migration ability of lung adenocarcinoma cells

LI Yuxuan, LI Kai, ZHANG Tongtong, GUO Yuanbiao, YIN Jun, BAO Yong

Respiratory Medicine of the Third People's Hospital of Chengdu, Chengdu 610031, Sichuan, China

Received:2016-11-14 Revised:2017-06-02 Online:2017-09-30 Published:2017-09-30

摘要/Abstract

摘要： 目的：研究热休克蛋白HDJ1在人肺腺癌组织、肺腺癌细胞株以及正常肺上皮细胞株中的表达情况，探讨HDJ1对肺腺癌细胞侵袭和迁移能力的影响。方法：采用免疫组织化学法检测HDJ1在人肺腺癌组织和癌旁正常组织中的表达情况；Western blot检测HDJ1在人肺腺癌细胞A549、H1299、H292及正常肺上皮细胞Bease-2B中的表达情况；使用pMagic 7.1-HDJ1-KD慢病毒转染A549细胞构建HDJ1稳定下调表达的细胞模型，并通过划痕实验、Transwell实验检测细胞的侵袭和迁移能力。结果：HDJ1在人肺腺癌组织中呈高表达；HDJ1在A549细胞中呈高表达；细胞划痕实验结果显示，与对照组细胞比较，HDJ1表达下调的A549细胞迁移率下降（P < 0.05）；Transwell实验结果表明，相对于对照组细胞，HDJI表达下调的A549细胞在侵袭和迁移实验中的穿膜细胞数均减少（P < 0.05）。结论：HDJ1在肺腺癌组织和细胞中均呈高表达，并促进肺腺癌细胞的侵袭和迁移能力。

关键词: 热休克蛋白, HDJ1, 肺腺癌, 侵袭, 迁移

Abstract: OBJECTIVE: To investigate expressions of HDJ1 in lung adenocarcinoma tissues and cell lines,and to reveal the role of HDJ1 in invasive ability of lung adenocarcinoma. METHODS: Immunohistochemistry method was used to detect expressions of HDJ1 in lung adenocarcinoma tissues and adjacent normal tissues. Western blot method was applied to determine expressions of HDJ1 in human lung adenocarcinoma cell lines (A549,H1299,H292) and normal lung epithelial cell line (Bease-2B). pMagic 7.1-HDJ1-KD lentivirus was transfected into the A549 cell line to construct a HDJ1 down-expressed cell line,and pMagic 7.1-NC lentivirus was transfected into the same cells as the control. The invasion ability of HDJ1 down-expressed group and control group was analyzed using the Transwell and the Wound Healing assays. RESULTS: The expression levels of HDJ1 in lung adenocarcinoma tissues were significantly higher than those in the adjacent normal tissues. HDJ1 was over-expressed in the A549 cell line. Compared with the control group,the Wound Healing assay results show that,the migration and healing ability of the HDJ1 down-expressed A549 cells were decreased. At 48 h after transfection,both the invasion and migration abilities of the HDJ1 down-expressed A549 cells were lower than that of control cells (P < 0.05). CONCLUSION: Heat shock protein HDJ1 were highly expressed in the lung adenocarcinoma tissues and cell lines. In addition,HDJ1 promoted the invasion and migration ability of lung adenocarcinoma cells.

Key words: heat shock protein, HDJ1, lung adenocarcinoma, invasion, migration

中图分类号:

R730.2

李雨轩, 李凯, 张彤彤, 郭元彪, 殷俊, 包勇. 热休克蛋白HDJ1 影响肺腺癌细胞侵袭和迁移能力的研究[J]. 癌变·畸变·突变, 2017, 29(5): 358-363.

LI Yuxuan, LI Kai, ZHANG Tongtong, GUO Yuanbiao, YIN Jun, BAO Yong. Heat shock protein HDJ1 on invasion and migration ability of lung adenocarcinoma cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 358-363.

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热休克蛋白HDJ1 影响肺腺癌细胞侵袭和迁移能力的研究

Heat shock protein HDJ1 on invasion and migration ability of lung adenocarcinoma cells

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