GMA诱导16HBE细胞恶性转化过程中LINC00472的表达及其意义

doi:10.3969/j.issn.1004-616x.2019.06.006

癌变·畸变·突变 ›› 2019, Vol. 31 ›› Issue (6): 449-453.doi: 10.3969/j.issn.1004-616x.2019.06.006

GMA诱导16HBE细胞恶性转化过程中LINC00472的表达及其意义

谢广云^1,3, 郭浩然², 王全凯^1,3, 马顺鹏^1,3, 宋佳阳¹, 乌瀚宝栎尔¹, 许建宁^1,3

1. 中国疾病预防控制中心职业卫生与中毒控制所, 北京 100050;
2. 江阴市疾病预防控制中心, 江苏无锡 214434;
3. 中国疾病预防控制中心化学污染与健康安全重点实验室, 北京 100050

收稿日期:2019-08-22 修回日期:2019-11-07 出版日期:2019-11-30 发布日期:2019-12-04
通讯作者: 许建宁,E-mail:jnx999@263.net E-mail:jnx999@263.net
作者简介:谢广云,E-mail:guangyunxie@163.com;郭浩然,guohaoran930807@outlook.com。
基金资助:
国家自然科学基金项目（81673221）

Differential expressions of LINC00472 during glycidyl methacrylate-induced malignant transformation of 16HBE cells

XIE Guangyun^1,3, GUO Haoran², WANG Quankai^1,3, MA Shunpeng^1,3, SONG Jiayang¹, WUHAN Baolier¹, XU Jianning^1,3

1. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050;
2. Jiangyin Municipal Center for Disease Control and Prevention, Wuxi 214434, Jiangsu;
3. Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China

Received:2019-08-22 Revised:2019-11-07 Online:2019-11-30 Published:2019-12-04

摘要/Abstract

摘要： 目的：探讨在甲基丙烯酸环氧丙酯（GMA）诱导人支气管上皮细胞（16HBE）恶性转化过程中长链非编码RNA LINC00472的表达特征及其生物学意义。方法：以DMSO作为溶剂对照组，实验组采用8 μg/mL的GMA染毒处理72 h，重复染毒3次，传代培养，收获两组细胞的第10、20和30代（分别代表前、中、后期）16HBE细胞，采用Arraystar人类LncRNA芯片（v4.0）分析细胞中LINC00472的表达改变，实时荧光定量PCR （qPCR）检测LINC00472和预测靶基因miR-24-3p的相对表达水平。结果：芯片检测结果显示，与同代龄DMSO对照组相比，GMA诱导16HBE细胞恶性转化过程中LINC00472在转化第10代和第20代分别下调2.30倍和3.57倍，第30代上调2.32倍。qPCR检测结果表明，与同代龄对照组相比，LINC00472在早期的相对表达水平差异无统计学意义，而在中期和后期的相对表达水平的变化情况与芯片结果基本一致，其可能的靶基因miR-24-3p在不同时期相对表达水平的差异均具有统计学意义（P < 0.05），但差异倍数均 < 2。结论：LINC00472在GMA诱导16HBE细胞恶性转化的前期表达水平未发生明显改变，而在细胞恶性转化的中期低表达、后期高表达，推测LINC00472可能在GMA诱导16HBE细胞恶性转化后期发挥作用，但LINC00472和miR-24-3p在此过程中是否存在相互作用以及其作用机制有待进一步研究。

关键词: 长链非编码RNA, LINC00472, 甲基丙烯酸环氧丙酯, 人支气管上皮细胞, 细胞恶性转化

Abstract: OBJECTIVE: To investigate the expression and biological significance of LINC00472 in the glycidyl methacrylate (GMA)-induced malignant transformation of the human bronchial epithelial cells (16HBE). METHODS: DMSO was used as the solvent control group,and the experimental group was treated with 8 μg/mL GMA for 72 hours,repeated for 3 times and then subcultured. The 10th,20th and 30th generation cells,corresponding to the pre-transformation,mid-term,and late period,were collected from the treated and the corresponding control groups. The Arraystar HumanLncRNA microarray was used to analyze the changes in expression of LINC00472 in the different cell generations. The prediction of target genes and their functions were conducted using bioinformatics databases. Real-time quantification PCR (qPCR) was used to detect the relative expression levels of LINC00472 and the predicted target genes. RESULTS: The microarray results show that LINC00472 in the 3 generations from the GMA-treated groups were:down-regulated by 2.30-fold,down-regulated by 3.57-fold,and up-regulated by 2.32-fold,respectively. The qPCR results confirmed that from the microarray analysis. In particular,the expression of LIN1000472 at the 10-generation was not statistically different from the control. Consistently,the relative expression of the target gene miR-24-3p was statistically significant (P < 0.05),but the multiples were less than 2-fold. CONCLUSION: Expression of LINC00472 in the GMA-induced malignant transformation of 16HBE cells increased from the early to the late cell generations. However,whether LINC00472 and miR-24-3p interact in this transformation process and their mechanisms of action need further study.

Key words: LncRNA, LINC00472, glycidyl methacrylate, human bronchial epithelial cells, malignant transformation

中图分类号:

R730.2

谢广云, 郭浩然, 王全凯, 马顺鹏, 宋佳阳, 乌瀚宝栎尔, 许建宁. GMA诱导16HBE细胞恶性转化过程中LINC00472的表达及其意义[J]. 癌变·畸变·突变, 2019, 31(6): 449-453.

XIE Guangyun, GUO Haoran, WANG Quankai, MA Shunpeng, SONG Jiayang, WUHAN Baolier, XU Jianning. Differential expressions of LINC00472 during glycidyl methacrylate-induced malignant transformation of 16HBE cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 449-453.

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GMA诱导16HBE细胞恶性转化过程中LINC00472的表达及其意义

Differential expressions of LINC00472 during glycidyl methacrylate-induced malignant transformation of 16HBE cells

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