新疆哈萨克族食管癌与正常组织间差异表达miRNA的筛查及验证

doi:10.3969/j.issn.1004-616x.2020.01.010

癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (1): 52-56.doi: 10.3969/j.issn.1004-616x.2020.01.010

新疆哈萨克族食管癌与正常组织间差异表达miRNA的筛查及验证

马莉莉¹, 张树丽², 李琴³, 许彭³, 封敏⁴, 李秀梅³

1. 新疆医科大学第五附属医院, 新疆乌鲁木齐 830011;
2. 新疆医科大学第一附属医院, 新疆乌鲁木齐 830011;
3. 新疆医科大学基础医学院, 新疆乌鲁木齐 830011;
4. 新疆医科大学附属肿瘤医院, 新疆乌鲁木齐 830011

收稿日期:2019-05-15 修回日期:2019-12-15 出版日期:2020-01-31 发布日期:2020-02-05
通讯作者: 李秀梅,E-mail:dr.lxm@163.com E-mail:dr.lxm@163.com
作者简介:马莉莉,E-mail:venuslilyma@163.com。
基金资助:
新疆维吾尔自治区自然科学基金项目（2017D01C180）

Screening and identification of differentially expressed miRNAs in esophageal cancers among Xinjiang Kazaks

MA Lili¹, ZHANG Shuli², LI Qin³, XU Peng³, FENG Min⁴, LI Xiumei³

1. The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011;
2. The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011;
3. Basic Medical College of Xinjiang Medical University, Urumqi 830011;
4. Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang, China

Received:2019-05-15 Revised:2019-12-15 Online:2020-01-31 Published:2020-02-05

摘要/Abstract

摘要： 目的：筛查并验证新疆哈萨克族食管癌组织与正常组织间差异表达的miRNAs，并初步探讨这些miRNAs在食管癌发生演进中的作用。方法：采用miRNA表达谱基因芯片分析系统筛查哈萨克族食管癌组织及远端正常食管组织中差异表达的miRNAs，实时荧光定量PCR检测验证差异显著miRNA的表达情况，分析miRNAs表达与食管癌临床病理指标的关系。结果：与远端正常组织相比较，miR-21、miR-132、miR-130b在哈萨克族食管癌组织中表达上调（P < 0.05），miR-141、miR-143、miR-133b、miR-195表达下调（P < 0.05）；miR-140表达变化无统计学意义；miR-21、miR-132、miR-143与食管癌临床分期有关，miR-21、miR-132、miR-143、miR-133b与淋巴结转移有关，miR-21、miR-141、miR-143、miR-133b与食管癌组织分化程度有关（P < 0.05）。结论：本研究采用基因芯片筛查出新疆哈萨克族食管癌组织与远端正常组织中差异表达的miRNAs，实时荧光定量PCR结果与基因芯片检测结果基本一致，miRNA表达的变化与新疆哈萨克族食管癌的发生发展有关。

关键词: 基因芯片, 食管癌, 微小RNA, 实时聚合酶链反应

Abstract: OBJECTIVE: To investigate differential expressions of microRNAs in Xinjiang Kazak's esophageal cancers and their involvement in cancers. METHODS: Expression of microRNAs were evaluated using microRNA microarray and qPCR in 47 cases of Kazak esophageal carcinoma tissues and distal cancer-free tissues. RESULTS: The results from the microRNA microarray and qPCR analyses were consistent with each other. Compared with the distal cancer-free tissues,expressions of miR-21,miR-132 and miR-130b were up-regulated in the esophageal carcinoma tissues while expressions of miR-141,miR-143,miR-195 and miR-133b were down-regulated,and the difference was significant (P < 0.05). miR-21,miR-132,miR-141 and miR-143 were related to the clinical stagings;miR-21,miR-132,miR-143 and miR-133b were related to lymph node metastasis;miR-21,miR-141,miR-143 and miR-133b were related to the differentiation of the cancers,and the differences were significant (P < 0.05). CONCLUSION: The microRNA microarray analyses were useful in screening out differentially expressed genes which were related to the occurrence of esophageal cancers while the microRNA analyses were useful in the occurrence of esophageal cancers.

Key words: gene chip, esophageal cancer, microRNA, real-time PCR

中图分类号:

R73-3

马莉莉, 张树丽, 李琴, 许彭, 封敏, 李秀梅. 新疆哈萨克族食管癌与正常组织间差异表达miRNA的筛查及验证[J]. 癌变·畸变·突变, 2020, 32(1): 52-56.

MA Lili, ZHANG Shuli, LI Qin, XU Peng, FENG Min, LI Xiumei. Screening and identification of differentially expressed miRNAs in esophageal cancers among Xinjiang Kazaks[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(1): 52-56.

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新疆哈萨克族食管癌与正常组织间差异表达miRNA的筛查及验证

Screening and identification of differentially expressed miRNAs in esophageal cancers among Xinjiang Kazaks

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