LncRNA CUST_49525和CUST_47163在急性髓系白血病患者外周血中的表达及其诊断意义

doi:10.3969/j.issn.1004-616x.2023.05.005

摘要/Abstract

摘要： 目的： 探讨外周血淋巴细胞中LncRNA CUST_49525和CUST_47163在急性髓系白血病(AML)中的表达及其诊断价值。方法： 采用实时荧光定量PCR(qPCR)检测AML患者与健康对照组人群外周血淋巴细胞中LncRNA CUST_49525和CUST_47163的表达水平，并通过胞质分裂阻滞微核试验检测两组人群淋巴细胞微核率。利用非参数Mann-Whitney U检验分析其差异，并绘制ROC曲线分析其诊断意义。结果： 与对照组比较，AML患者外周血淋巴细胞中LncRNA CUST_49525和CUST_47163的表达水平均下调(P<0.01)，而淋巴细胞微核率显著升高(P<0.01)。ROC曲线分析结果显示，LncRNA CUST_49525在外周血淋巴细胞中表达的ROC曲线下面积为0.742，其95%CI为0.627~0.858；LncRNA CUST_47163的ROC曲线下面积为0.859，其95%CI为0.769~0.949。外周血淋巴细胞微核细胞率的ROC曲线下面积为0.948，其95%CI为0.899~0.997。三者联合诊断的ROC曲线下面积为0.970，95%CI为0.931~1.000。与对角线下面积(0.500)相比，CUST_49525、CUST_47163、微核率和联合诊断各组AUC均增大，差异具有统计学意义(P<0.01)，且联合诊断效果优于单一指标诊断，单一指标诊断中微核率优于其他指标(P<0.01)。结论： AML患者外周血淋巴细胞中LncRNA CUST_49525和CUST_47163表达水平均下调，二者与淋巴细胞微核率的联合诊断效果优于单一指标，可作为AML诊断的潜在参考指标。

关键词: 急性髓系白血病, 长链非编码RNA, 微核, 诊断

Abstract: OBJECTIVE:To investigate expression and diagnostic values of LncRNA CUST_49525 and CUST_47163 in peripheral blood lymphocytes in patients with acute myeloid leukemia (AML). METHODS: Real time fluorescence quantitative PCR (qPCR) was used to detect expression levels of LncRNA CUST_49525 and CUST_47163 in peripheral blood lymphocytes of patients and healthy controls. The cytokinesis-arrest micronucleus test was used to detect micronuclei levels in lymphocytes. The collected data were evaluated using the non-parametric Mann Whitney U test to determine significant differences and ROC curves were drawn to determine diagnostic significance. RESULTS: Compared with the control group,the expression levels of both LncRNA CUST_49525 and CUST_47163 from the patients were significantly down-regulated (P<0.01), while the micronucleus rates were significantly increased (P<0.01). The ROC curve analyses show that the area under the ROC curve of LncRNA CUST_49525 in the patients was 0.742,with a 95%CI of 0.627-0.858;and that for LncRNA CUST_47163 was 0.859,with a 95%CI of 0.769-0.949. The area under the ROC curve for the micronucleus rates was 0.948,with a 95%CI of 0.899-0.997 (P<0.01). The area under the ROC curve for the combined diagnosis of the three was 0.970,with a 95%CI of 0.931-1.000 (P<0.01). CONCLUSION: The expression levels of LncRNA CUST_49525 and CUST_47163 in peripheral blood lymphocytes of AML patients were abnormally down-regulated. The combined diagnostic effect of the two together with the micronucleus rate was superior to a single indicator,and can be a potential reference indicator for AML diagnosis.

Key words: acute myeloid leukemia, LncRNA, micronucleus, diagnosis

中图分类号:

R733.71

高冉, 哈丽亚·塔力达吾别克, 刘晓东, 田薇. LncRNA CUST_49525和CUST_47163在急性髓系白血病患者外周血中的表达及其诊断意义[J]. 癌变·畸变·突变, 2023, 35(5): 355-359.

GAO Ran, HALIYA Talidawubieke, LIU Xiaodong, TIAN Wei. Expression and diagnostic significance of LncRNA CUST_49525 and CUST_47163 in peripheral blood of patients with acute myeloid leukemia[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(5): 355-359.

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