发育相关基因Ercc6l的分子克隆与表达分析及其在酒精致畸中的作用

doi:10.3969/j.issn.1004-616x.2004.02.001

癌变·畸变·突变 ›› 2004, Vol. 16 ›› Issue (2): 65-74.doi: 10.3969/j.issn.1004-616x.2004.02.001

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发育相关基因Ercc6l的分子克隆与表达分析及其在酒精致畸中的作用

许雅君;陈祥贵;李　勇

北京大学公共卫生学院营养与食品卫生学系，北京大学分子毒理与发育分子生物学实验室，北京　100083

收稿日期:2003-12-02 修回日期:2004-02-15 出版日期:2004-03-30 发布日期:2004-03-30
通讯作者: 李　勇

Molecula Cloning,Characterization and Tissue Expression of Ercc6l, a novel Development Related Gene that Contributes to the Teratogenic Effect of Alcohol

XU Ya-jun;CHEN Xiang-gui;LI Yong

Department of Nutrition ＆ Food Hygiene, School of Public Health, Laboratory of Molecular toxicology ＆ Developmental Molecular Biology, Peking University, Beijing 100083, China

Received:2003-12-02 Revised:2004-02-15 Online:2004-03-30 Published:2004-03-30
Contact: LI Yong

摘要/Abstract

摘要： 背景与目的：在GeneBank中筛选新的小鼠发育相关基因，研究其在酒精致畸中的作用。材料与方法：利用EST介导的基因克隆和表达谱分析进行新基因克隆、表达和同源性分析；利用RT－PCR、绿色荧光蛋白标记基因转染、全胚胎原位杂交、石蜡切片原位杂交和Northern杂交进行新基因的验证、亚细胞和组织定位及其在酒精致畸中的表达变化研究。结果：成功克隆小鼠基因Ercc6l，其在胚胎期正常组织中强表达，在新生鼠表达明显减弱，在成年鼠几乎不表达。酒精染毒体内、外实验均显示畸变胚胎Ercc6l的表达显著降低。结论： Ercc6l是小鼠胚胎发育相关基因，同时是酒精致畸作用的分子靶点。

关键词: Ercc6l, 基因克隆, SNF2蛋白, 酒精

Abstract: BACKGROUND ＆ AIM: Molecular cloning and expression analysis of a novel mouse development related gene, and exploring its role in the teratogenic action of alcohol. MATERIAL AND METHODS: EST assembly was used in gene cloning, expression and homology analysis; RT－PCR, transfection of fused green fluorescent protein, whole mount in situ hybridization, section in situ hybridization and northern blot were used in gene verification, subcellular and tissue localization and expression difference between normal and alcohol treated embryos both in vivo and in vitro. RESULTS: Successfully cloned a novel mouse development related gene, Ercc6l. It expresses strongly in normal tissues of mouse embryos, but is significantly down regulated in neonates and hardly expresses in adults. Expression of Ercc6l in alcohol treated mouse embryos was significantly down regulated compared to their paired controls. CONCLUSION: Ercc6l is a novel development related gene of mice, as well as one of the molecular targets of alcohol teratogenic action.

Key words: Ercc6l, gene cloning, SNF2 protein, alcohol　

许雅君, 陈祥贵, 李　勇. 发育相关基因Ercc6l的分子克隆与表达分析及其在酒精致畸中的作用[J]. 癌变·畸变·突变, 2004, 16(2): 65-74.

XU Ya-jun, CHEN Xiang-gui, LI Yong. Molecula Cloning,Characterization and Tissue Expression of Ercc6l, a novel Development Related Gene that Contributes to the Teratogenic Effect of Alcohol[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2004, 16(2): 65-74.

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发育相关基因Ercc6l的分子克隆与表达分析及其在酒精致畸中的作用

Molecula Cloning,Characterization and Tissue Expression of Ercc6l, a novel Development Related Gene that Contributes to the Teratogenic Effect of Alcohol

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