川芎提取物的多模型体系抗氧化活性测定

doi:10.3969/j.issn.1004-616x.2011.02.002

›› 2011, Vol. 23 ›› Issue (2): 87-092.doi: 10.3969/j.issn.1004-616x.2011.02.002

川芎提取物的多模型体系抗氧化活性测定

李玉亮，彭洁，梁欣，海春旭*

第四军医大学军事预防医学系毒理学教研室，陕西西安 710032

收稿日期:2010-04-06 修回日期:2010-11-11 出版日期:2011-03-30 发布日期:2011-03-30
通讯作者: 海春旭

Antioxidation of Szechwan Lovage Rhizome extracts in vivo and in vitro lipid peroxidation models

LI Yu-liang,PENG Jie,LIANG Xin，HAI Chun-xu*

Department of Toxicology, School of Military Preventive Medicine, the Fourth Military Medical University，Xi’an 710032, China

Received:2010-04-06 Revised:2010-11-11 Online:2011-03-30 Published:2011-03-30
Contact: HAI Chun-xu

摘要/Abstract

摘要： 研究川芎醇提物和水提物在几种自由基体外模型中的抗氧化作用位点与活性强度，以及此两种提取物在小鼠氧化损伤模型中的综合抗氧化作用。方法：建立DPPH体系，观察川芎醇提物和水提物对DPPH自由基的清除率；建立·OH和O2-. 化学发光体系观察川芎的两种提取物对发光强度的抑制作用；采用CHP、Vc /Fe2+和CC14/NADP+作为激发剂，建立微粒体脂质过氧化(LPO)损伤模型，观察川芎两种提取物对MDA生成的抑制作用。建立小鼠氧化损伤模型，观察川芎两种提取物对SOD和CAT蛋白表达水平的影响，以及对MDA生成的抑制作用。结果：在DPPH体系中，川芎的醇和水两种提取物对DPPH的清除率均显著高于对照组(P<0.01)，且呈明显剂量-效应关系，川芎醇提物和水提物对DPPH的IC50分别为3.47和6.19 mg/ml；在两种化学发光体系中，川芎的醇提物和水提物对O2-.和·OH的清除率均显著高于对照组(P<0.01)，且呈明显剂量-效应关系，川芎醇提物对O2-.和·OH的IC50分别为2.56和4.21 mg/ml，水提物对O2-.和·OH为2.34和2.80 mg/ml；3种LPO损伤模型中川芎两种提取物各浓度组的MDA含量均显著低于对照组(P<0.05)；在体内实验中，与对照组相比，川芎水提物和醇提物组均明显降低肝组织和血清内MDA的含量(P<0.05)；川芎水提物和醇提物能明显提高肝组织内SOD蛋白表达水平；川芎醇提物可以提高肝组织内CAT蛋白表达水平。结论：川芎提取物在几种体外模型和体内模型中均具有较好的抗氧化作用。

关键词: 川芎, DPPH, 化学发光, 脂质过氧化, 抗氧化作用

Abstract: To study the antioxidative characteristics and activities of the ethanol and water extracts of Szechwan Lovage Rhizome(SLR) in several free radical models in vitro and in oxidative- damage model in mice. METHODS： The DPPH system model was applied to observe the inhibitory rates of DPPH radicals by different concentrations of SLR extracts. The chemiluminescence system models for determination of ·OH and O2-. were built to observe the inhibitory rates of luminous intensity by different concentrations of SLR extracts. The microsomal lipid peroxidation model stimulated by CHP, Vc /Fe2+ and CC14/NADP+ was established to observe the inhibition of MDA by different concentrations of SLR extracts. The oxidative-damage model in mice was built to observe the inhibitory rates of MDA and changes of liver protein CAT and SOD expressions. RESULTS: In the DPPH system, the inhibitory rates of DPPH radicals by the two SLR extracts were significantly higher than that in control group，with an evident dose-effect relationship. The IC50 of DPPH radicals by the ethanol and water extracts of SLR were 3.47 mg/ml and 6.09 mg/ml, respectively. In the two chemiluminescence systems, the inhibitory rates of ·OH and O2-. by different concentrations of SLR extracts were significantly higher than that in control group again with an evident dose-effect relationship. The IC50 of O2-. and ·OH by the ethanol extracts of SLR were 2.56 mg/ml and 4.21 mg/ml, respectively, and the IC50 were 2.34 mg/ml and 2.80 mg/ml, respectively, by the water extracts. Compared with that in control group, the content of MDA in the three LPO models was decreased significantly(P<0.05) at different concentrations and had a dose-effect relationship. In vivo studies, after SLR oral administration, decrease of MDA was observed in liver and serum. The expressions of SOD and CAT were increased by ethanol extracts. Water extracts only increased the expression of SOD but that of CAT was not altered. CONCLUSION：In several in vitro and in vivo models described above, the ethanol and water extracts of SLR exhibited effective antioxidative effects.

Key words: Szechwan Lovage Rhizome, DPPH, chemiluminescence, lipid peroxidation antioxidation

李玉亮，彭洁，梁欣，海春旭*. 川芎提取物的多模型体系抗氧化活性测定[J]. , 2011, 23(2): 87-092.

LI Yu-liang,PENG Jie,LIANG Xin，HAI Chun-xu*. Antioxidation of Szechwan Lovage Rhizome extracts in vivo and in vitro lipid peroxidation models[J]. , 2011, 23(2): 87-092.

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川芎提取物的多模型体系抗氧化活性测定

Antioxidation of Szechwan Lovage Rhizome extracts in vivo and in vitro lipid peroxidation models

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