›› 2011, Vol. 23 ›› Issue (3): 209-212.doi: 10.3969/j.issn.1004-616x.2011.03.012

• 论著 • 上一篇    下一篇

一次性筷子、牙签及纸杯浸提液对小鼠L-929细胞的毒性作用

陈思凡1，2,李文学1,贾慧影2,陈建玲1，2,杨光宇1,朱 伟1，2，*   

  1. 1. 广州市疾病预防控制中心，广东 广州 510080； 2. 中山大学公共卫生学院，广东 广州 510080
  • 收稿日期:2010-12-22 修回日期:2011-01-19 出版日期:2011-05-30 发布日期:2011-05-30
  • 通讯作者: 朱 伟

Toxic effects of the extracted solution of disposable chopstick toothpick or cup in murine L-929 cells

CHEN Si-fan1，2，LI Wen-xue1，JIA Hui-ying2，CHEN Jian-ling1，2，YANG Guang-yu1，ZHU Wei1，2，*   

  1. 1. Guangzhou Center for Disease Control and Prevention, Guangzhou 510080; 2. School of Public Health, Sun Yat-sen University, Guangzhou 510080, Guangdong, China
  • Received:2010-12-22 Revised:2011-01-19 Online:2011-05-30 Published:2011-05-30
  • Contact: ZHU Wei

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摘要： 评价一次性筷子、牙签及纸杯浸提液对小鼠L-929细胞的毒性作用。方法：参照国家标准，制备一次性筷子、牙签及纸杯等样品浸提液，采用样品浸提液配制培养液培养小鼠L-929细胞，在光镜下观察细胞生长形态，采用细胞计数法计算细胞失贴壁率，采用WST-1法测定细胞活性并计算细胞增殖度。 结果：与对照组相比，采用浸提液配制的细胞培养液培养细胞4 d或7 d后，各组细胞出现凋亡或坏死征象，细胞失贴壁率明显升高 (P<0.05)，细胞活性及细胞增殖度明显下降 (P<0.05)。 结论：一次性筷子、牙签及纸杯用具等能影响L-929细胞生长及增殖，可能具有潜在的毒性作用。

关键词: 牙签, 一次性筷子, 一次性纸杯, 细胞毒性

Abstract: To evaluate the toxic effects of the extracted solution of disposable chopstick， toothpick or cup in murine L-929 cells. METHODS：According to the national standards，we prepared cell culture medium with the extracted solution of disposable chopsticks，toothpicks or cups. The cell morphology of murine L-929 cells was examined microscopically and cell viability was detected with WST-1 staining method. The detached cell ratios and the relative growth rate of L-929 cells were calculated. RESULTS：Compared with the control，after treatment for 4 days or 7 days，cells in the experimental groups showed signs of apoptosis or necrosis. The detached cell ratios were increased significantly (P<0.05)，while cell viability and relative growth rate declined significantly (P<0.05). CONCLUSION： Treatments with extracted solution of disposable chopstick，toothpick or cup could affect cell growth and proliferation，thus probably possess potential toxic effects.

Key words: toothpick, disposable chopstick, disposable cup, cytotoxicity

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