慢性酒精中毒及酒精戒断后小鼠肝细胞IL-28和mac-3的表达

doi:10.3969/j.issn.1004-616x.2011.05.011

›› 2011, Vol. 23 ›› Issue (5): 370-373.doi: 10.3969/j.issn.1004-616x.2011.05.011

慢性酒精中毒及酒精戒断后小鼠肝细胞IL-28和mac-3的表达

常晓兰,黄东阳,冯广友，*

1. 汕头大学医学院生物学教研室；2. 汕头大学医学院病理生理学教研室，广东汕头 515041

收稿日期:2011-06-17 修回日期:2011-07-30 出版日期:2011-09-30 发布日期:2011-09-30
通讯作者: 冯广友

Expressions of IL-28 and mac-3 in liver of mice with chronic alcoholism and alcohol withdrawal

CHANG Xiao-lan，HUANG Dong-yang，FENG Guang-you，*

1. Department of Cellular Biology, Shantou University Medical College; 2. Department of Pathophysiology, Shantou University Medical College, Shantou 515041, China

Received:2011-06-17 Revised:2011-07-30 Online:2011-09-30 Published:2011-09-30
Contact: FENG Guang-you

摘要/Abstract

摘要： 目的：观察慢性酒精中毒及酒精戒断后小鼠肝细胞IL-28和mac-3的表达。方法：昆明小鼠随机分为酒精灌胃不同时间 (4、8、12周)的3个实验组，每组16只，同时设对照组 (8只)。各实验组小鼠每天用40%医用酒精按8 mg/g剂量灌胃，对照组则灌胃等量生理盐水。各组小鼠分别于第4、 8、12周末取半数处死，剖取肝脏，其余半数小鼠延时24 h处死，在延时期间不再酒精灌胃。用HE染色观察肝组织病理学改变，用免疫组化方法检测肝细胞中IL-28和mac-3的表达。结果：灌胃4、8、12周实验组小鼠肝组织均出现脂肪变性、炎性改变及肝纤维化等慢性酒精性肝病的表现，且随灌胃时间的延长病变加重，而对照组则未出现此种改变。各实验组肝细胞IL-28和mac-3的表达较对照组减弱(P均＜0.05)，但在酒精戒断后的表达增强，与各组戒断前的差异均有统计学意义(P＜0.05)。结论：慢性酒精中毒造成肝枯否细胞损伤，酒精戒断后在肝内可能发生一过性炎症反应。

关键词: 酒精中毒, 酒精戒断, IL-28, mac-3

Abstract: OBJECTIVE: To investigate the expressions of IL-28 and mac-3 in liver of chronic alcoholism mice and withdraw from alcohol. METHODS：The dose of 40% alcohol [8 mg/ (g·d)] was administered via gastrolavage once daily for 12 weeks in Kunming mice. The control mice were given the same volume of saline. The experimental mice were sacrified at the end of 4th，8th，12th week and after withdrawal from alcohol 24 hours. Pathologic changes of liver were examined under light microscope after HE staining，expressions of IL-28 and mac-3 were examined by immunohistochemitry. RESULTS：The tissues of treated mice showed various changes of chronic alcoholic hepatitis at the end of 4，8，12 week，such as fatty degerneration，inflammatory and fibrosis，more severe with longer alcohol administration. The expressions of IL-28 and mac-3 were decreased in alcoholic mice but increased in mice withdrawal from alcohol. CONCLUSION：Alcohol could injure Kupffer cells or inhibit their functions. Withdrawal of alcohol may introduce the rebound of IL-28 and mac-3 expressions or temporary inflammation of liver.

Key words: alcoholism, withdrawal from alcohol, IL-28, mac-3

常晓兰,黄东阳,冯广友，*. 慢性酒精中毒及酒精戒断后小鼠肝细胞IL-28和mac-3的表达[J]. , 2011, 23(5): 370-373.

CHANG Xiao-lan，HUANG Dong-yang，FENG Guang-you，*. Expressions of IL-28 and mac-3 in liver of mice with chronic alcoholism and alcohol withdrawal[J]. , 2011, 23(5): 370-373.

慢性酒精中毒及酒精戒断后小鼠肝细胞IL-28和mac-3的表达

Expressions of IL-28 and mac-3 in liver of mice with chronic alcoholism and alcohol withdrawal

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