›› 2012, Vol. 24 ›› Issue (2): 145-147.doi: 10.3969/j.issn.1004-616x.2012.02.015

• 检测研究 • 上一篇    下一篇

叶酸对乙醇诱发的人成淋巴细胞遗传损伤的影响

陈海燕，倪 娟，汪 旭   

  1. 1. 云南师范大学生命科学学院 ，云南 　 昆明 　 650092； 2. 云南师范大学生物能源持续开发与利用教育部工程研究中心，云南 昆明 650092
  • 收稿日期:2011-08-17 修回日期:2011-11-30 出版日期:2012-03-30 发布日期:2012-03-30
  • 通讯作者: 汪 旭

The effect of folic acid on genetic damage induced by ethanol in human lymphoblast cell line

CHEN Hai-yan，NI Juan，WANG Xu   

  1. 1. School of Life Sciences, Yunnan Normal University, Kunming 650092; 2. The Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650092, Yunnan, China
  • Received:2011-08-17 Revised:2011-11-30 Online:2012-03-30 Published:2012-03-30
  • Contact: WANG Xu

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摘要： 目的： 探讨叶酸 (folic acid，FA)对乙醇 (ethanol，EtOH)诱发的人成淋巴细胞遗传损伤的影响。方法：人成淋巴细胞株GM12593培养在含有不同FA浓度 (20、200、2 000 nmol/L)的RPMI-1640培养基中，并同时在每种FA浓度的培养体系中分别加入0.09%、0.36%和1.34% (V/V)的EtOH，8 d后用细胞松弛素B (cytochalasin B，CB)处理28 h，胞质阻断微核细胞组分析 (cytokinesis-block micronucleus cytome assay，CBMN Cyt)比较不同培养条件下的细胞遗传损伤，包括微核化双核细胞 (micronucleated binucleated cell，MNed BNC)、核芽 (nuclear bud，BUD)、核质桥 (nucleoplasmic bridges，NPB)的频率差异。 结果：乙醇在受试细胞中诱发 MNed BNC、BUD和NBP频率显著升高 (P<0.01)；2 000 nmol/L的叶酸能显著降低由乙醇诱发的MNed BNC、BUD和NBP频率 (P<0.01)。结论：乙醇对细胞遗传物质具有损伤效应，叶酸对乙醇的遗传毒性具有防护效应。

关键词: 叶酸, 乙醇, 遗传损伤, 胞质阻断微核细胞组分析

Abstract: OBJECTIVE: The study focused on the effect of folic acid (FA) on genetic damage induced by ethanol (EtOH) in human lymphoblast cell line. METHODS：The human lymphoblast cell line GM12593 were cultured in RPMI-1640 medium modified with different concentrations of FA (20，200，2 000 nmol/L). Ethanol was added into the medium at the concentrations of 0.09%，0.36% and 1.34% (V/V) through the culture period. After 8 days culture，the cytokinesis-block micronucleus assay (CBMN) was used to evaluate frequencies of micronucleated binucleated cell (MNed BNC)，nuclear buds (BUD) and nucleoplasmic bridges (NPB) in binucleated cells. RESULTS：Ethanol significantly increased the frequencies of MNed BNC，BUD and NPB (P<0.01). A concentration of 2 000 nmol/L of FA reduced the genotoxic effects from ethanol significantly (P <0.01). CONCLUSION：Ethanol could induce DNA damage，folic acid was able to protect human from the genotoxicity induced by enthnol in vitro.

Key words: folic acid, ethanol, genetic damage, cytokinesis-block micronucleus cytome assay

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