›› 2012, Vol. 24 ›› Issue (4): 305-308,.doi: 10.3969/j.issn.1004-616x.2012.04.016

• 检测研究 • 上一篇    下一篇

二- (2，4-二氯苯甲酰异羟肟酸)二苯基合锡的遗传毒性研究

秦秀军;岳 娟;李曙芳;尹晶晶;许文黎;郑 娟;李云兰;李青山   

  1. ( 1. 中国辐射防护研究院，山西 太原 030006；2. 山西医科大学药学院，山西 太原 030001 )
  • 收稿日期:2012-01-11 修回日期:2012-03-08 出版日期:2012-07-30 发布日期:2012-07-30
  • 通讯作者: 李青山

Genotoxicity of di-phenyl-di- (2, 4 - chlorobenzohydroxamato) tin

QIN Xiu-jun;YUE Juan;LI Shu-fang;YIN Jing-jing;XU Wen-li;ZHENG Juan;LI Yun-lan;LI Qing-shan   

  1. ( 1. China Institute for Radiation Protection, Taiyuan 030006; 2. School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, Shanxi, China)
  • Received:2012-01-11 Revised:2012-03-08 Online:2012-07-30 Published:2012-07-30
  • Contact: LI Qing-shan

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摘要： 目的： 研究二- (2，4-二氯苯甲酰异羟肟酸)二苯基合锡 [di-phenyl-di- (2，4-chlorobenzohydroxamato) tin，DPDCT]是否存在遗传毒性。方法：应用Ames试验、中国仓鼠肺细胞 (Chinese hamster lung，CHL)染色体畸变试验和小鼠骨髓嗜多染红细胞微核试验，研究DPDCT的遗传毒性。结果：Ames试验中，DPDCT在每皿500、5 000 μg剂量时，诱发TA97 (±S9)、TA98 (±S9)、TA100 (±S9)及TA1535 (＋S9)菌株产生的回变菌落数明显高于阴性对照组 (P＜0.01)；染色体畸变试验中，DPDCT在4.00 μg/ml浓度时，在-S9条件下作用24 h后，CHL细胞染色体畸变率明显高于溶剂对照组 (P＜0.01)； 微核试验中，DPDCT在12.5~50.0 mg/kg浓度范围内，无致小鼠骨髓嗜多染红细胞微核形成作用。结论：DPDCT在体外试验中，具有遗传毒性，但在小鼠体内试验中，未发现遗传毒性。

关键词: 二- (2, 4-二氯苯甲酰异羟肟酸)二苯基合锡, 遗传毒性, Ames试验, 染色体畸变试验, 微核试验

Abstract: OBJECTIVE: To investigate the genotoxicity of di-phenyl-di- (2，4- chlorobenzohydroxamato) tin (DPDCT). METHODS：Ames test，chromosomal aberration test in Chinese hamster lung(CHL) cell and micronucleus test in mouse bone marrow were carried out. RESULTS：In Ames test，at concentration of 500 and 5 000 μg/plate，the reversal colonies in TA97 (±S9)，TA98 (±S9) ，TA100 (±S9) and TA1535 (+S9) were significantly increased (P＜0.01) compared with negative control. At concentration of 4.00 μg/ml，DPDCT induced a significant increase in number of chromosomal aberrations (P＜0.01) compared with solvent control at 24 h after administration without metabolic activation. Micronucleus test indicated DPDCT had no mutation effect on the rate of mouse micronucleated polychromatic erythrocytes at dose of 12.5-50.0 mg/kg. CONCLUSION：DPDCT showed genotoxicity in vitro，but not in vivo.

Key words: di-phenyl-di- (2, 4-chlorobenzohydroxamato) tin, genotoxicity, ames test, chromosomal aberration test, micronucleus test

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