PCD检测方法的建立及其在研究外来激素对大鼠睾丸细胞PCD影响的应用

doi:10.3969/j.issn.1004-616x.1997.01.008

癌变·畸变·突变 ›› 1997, Vol. 09 ›› Issue (1): 23-28.doi: 10.3969/j.issn.1004-616x.1997.01.008

PCD检测方法的建立及其在研究外来激素对大鼠睾丸细胞PCD影响的应用

祝红红　黄幸纾

浙江医科大学卫生毒理学教研室　杭州　310031

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-01-30 发布日期:1997-01-30

ESTABL ISHM ENT OF A M ETHOD FOR ID ENT IFY ING PCD AND ITS USE IN STUDY ING THE EFFECTS OF GNRH- A ON PCD IN RAT TEST IS GERM CELLS

Zhu Honghong, Huang Xingshu

Toxicology Department of Zhejiang Medical University ,Hangzhou 310031

Received:1900-01-01 Revised:1900-01-01 Online:1997-01-30 Published:1997-01-30

摘要/Abstract

摘要： 本研究1.建立了检测PCD的石蜡切片原位末端标记( ISEL ) 法, 在石蜡切片原位用免疫组化法标记核DNA,使 PCD 细胞呈黄色或棕黄色的阳性着染, 在普通光学显微镜下即可鉴别。本实验用加热和蛋白酶K同时处理背景, 使非特异性染色几乎不存在; 使用过氧化物酶—显色系统缩短时间2小时,提高了筛检DAB 速度。2.应用ISEL法研究外来激素对大鼠睾丸细胞的影响,结果发现GnRH-A是一个促凋原,具有明显的生殖毒性。

关键词: 编程性细胞死亡(PCD ), 原位末端标记, 睾丸细胞, 垂体促性腺激素释放激素(GnRH -A)

Abstract: In order to evaluate the reproductive toxicity of environmental horm ones and explore them echanism of male infertility,the present experiment used in situ ending labeling (ISEL) assay to analyse the effects of environmental chemicals on apoptosis Firstly established the method of ISEL ,and secondly used the ISEL to measure the reproduction tox icity of GnRH-A.ISEL has the advantages of high specifity and sensitivity, and good repetition. In situ analysis of developing test is demonstrated that sperm atocytes and sperm atogon ia constituted the major PCD cell types That PCD rate in GnRHA group was apparently higher than that of the control group showed that GnRH -A is an apogen exerting reproduction toxicity.

Key words: Programmed cell death (PCD), In situ end labeling (ISEL) Testis germ cell, Gonadotroping releasing hormone analogue(GnRH -A )

祝红红　黄幸纾. PCD检测方法的建立及其在研究外来激素对大鼠睾丸细胞PCD影响的应用[J]. 癌变·畸变·突变, 1997, 09(1): 23-28.

Zhu Honghong, Huang Xingshu. ESTABL ISHM ENT OF A M ETHOD FOR ID ENT IFY ING PCD AND ITS USE IN STUDY ING THE EFFECTS OF GNRH- A ON PCD IN RAT TEST IS GERM CELLS[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1997, 09(1): 23-28.

PCD检测方法的建立及其在研究外来激素对大鼠睾丸细胞PCD影响的应用

ESTABL ISHM ENT OF A M ETHOD FOR ID ENT IFY ING PCD AND ITS USE IN STUDY ING THE EFFECTS OF GNRH- A ON PCD IN RAT TEST IS GERM CELLS

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