刺五加提取物在自由基模型中的活性作用

doi:10.3969/j.issn.1004-616x.2009.02.012

癌变·畸变·突变 ›› 2009, Vol. 21 ›› Issue (2): 127-131.doi: 10.3969/j.issn.1004-616x.2009.02.012

刺五加提取物在自由基模型中的活性作用

王　欣, 海春旭, 梁　欣

第四军医大学军事预防医学系毒理学教研室，陕西　西安　710032

收稿日期:2008-09-11 修回日期:2008-11-14 出版日期:2009-03-30 发布日期:2009-03-30

Activities of Acanthopanax Extracts in Vitro in Free Radical Models

WANG Xin，HAI Chun-xu, LIANG Xin

Department of Toxicology，School of Military Preventive Medicine, the Fourth Military Medical University，Xi'an 710032, China

Received:2008-09-11 Revised:2008-11-14 Online:2009-03-30 Published:2009-03-30

摘要/Abstract

摘要： 背景与目的：研究刺五加醇提物和水提物在几种自由基体外模型中的抗氧化作用。材料与方法：建立DPPH体系，观察刺五加不同浓度的醇提物和水提取物对DPPH自由基的抑制作用；建立·OH和O2 两种化学发光体系观察刺五加不同浓度的两种提取物对发光强度的抑制作用；采用CHP、VC／Fe2＋和CC14／NADP作为激发剂，建立微粒体脂质过氧化(LPO)损伤模型，观察不同浓度的刺五加两种提取物对MDA生成的抑制作用。结果：在DPPH体系中，刺五加两种提取物对DPPH的抑制率均显著高于对照组 (P<0.01)，呈明显剂量-效应关系，刺五加醇提物和水提物对DPPH的IC50分别为0.18 mg/ml和0.09 mg/ml；在两种化学发光的体系中，除了0.625 mg/ml的刺五加醇提物与对照组的差异无统计学意义(P=0.102)，其它浓度的两种提取物对O2 和·OH的抑制率均显著高于对照组(P<0.01)，呈明显剂量-效应关系，刺五加醇提物的IC50分别为0.488 mg/ml和1.29 mg/ml；水提物为 0.24 mg/ml和0.37 mg/ml；在CHP模型中，1.25 mg/ml的刺五加水提物表现出了一定的促氧化作用，但差异无统计学意义 (P>0.05)。3种LPO损伤模型中刺五加两种提取物各浓度组的MDA含量均显著低于对照组(P<0.05)，且有一定的剂量-效应关系。结论：刺五加在几种体外模型中均具有较强的抗氧化作用，在DPPH和两种化学发光体系中，刺五加水提物的抗氧化活性强于醇提物；而在3种LPO模型中，刺五加醇提物的抗氧化活性强于水提物。

关键词: 刺五加, DPPH自由基, 化学发光, 抗氧化作用, 脂质过氧化

Abstract: BACKGROUND AND AIM: To study the antioxidative activities of different concentrations of ethanol and water extracts of Acanthopanax in several free radical models in vitro and their dose-effect relationships. MATERIALS AND METHODS: The DPPH system model was built to study the inhibitive rates of DPPH radicals by different concentrations of Acanthopanax extracts. Two chemiluminescence system models for determination of ·OH and O2 were set up to examine the inhibitive rates of luminous intensity by different concentrations of Acanthopanax extracts. The lipid microsomal peroxidation model stimulated by CHP, VC／Fe2＋ and CC14／NADP were used to assess the inhibitive rates of MDA by different concentrations of Acanthopanax extracts. RESULTS: In the DPPH system, the inhibitive rates of DPPH radicals by the two Acanthopanax extracts were significantly higher than that in control group which showed an evident dose-effect relationship. The IC50 of DPPH radicals by the ethanol and water extracts of Acanthopanax was 0.18 mg/ml and 0.09 mg/ml, respectively. In the two chemiluminescence systems, except the ethanol extract of 0.625 mg/ml (P=0.102), the inhibitive rates of ·OH and O2 by different concentrations of Acanthopanax extracts were significantly higher than that in control group and showed an evident dose-effect relationship. The IC50 of O2 and ·OH by ethanol extracts of Acanthopanax were 0.488 mg/ml and 1.29 mg/ml, respectively, and the IC50 were 0.24 mg/ml and 0.37 mg/ml, respectively, by the water extracts. In the CHP model, the water extracts of Acanthopanax showed an activity of promoting oxidation when the concentration was 1.25 mg/ml. But the variance didn't reach statistical significance. Compared with that in control group, the content of MDA in the three LPO models decreased significantly(P<0.05) at different concentrations and had a dose-effect relationship. CONCLUSION: In several in vitro models described above, the ethanol and water extracts of Acanthopanax demonstrated effective antioxidative effects. In DPPH and two chemiluminescence systems, the antioxidative activities of the water extracts were stronger than that of the ethanol extracts; whilst in the three LPO models, the results were opposite.

Key words: acanthopanax, DPPH, chemiluminescence, antioxidative activities, lipid peroxidation

王　欣, 海春旭, 梁　欣. 刺五加提取物在自由基模型中的活性作用[J]. 癌变·畸变·突变, 2009, 21(2): 127-131.

WANG Xin, HAI Chun-xu, LIANG Xin. Activities of Acanthopanax Extracts in Vitro in Free Radical Models[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2009, 21(2): 127-131.

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刺五加提取物在自由基模型中的活性作用

Activities of Acanthopanax Extracts in Vitro in Free Radical Models

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