癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (2): 81-085.doi: 10.3969/j.issn.1004-616x.2010.02.001

• 论著 •    下一篇

三氯乙烯对L-02肝细胞中SET表达的影响

蒋英芝1;2;刘建军2;周桂凤1;张兵2;郑波2;黄爱君2   

  1. 1.湖南师范大学医学院预防医学系，湖南 长沙 410006； 2. 深圳市疾病预防控制中心，广东 深圳 518020
  • 收稿日期:2009-10-30 修回日期:2009-12-01 出版日期:2010-03-30 发布日期:2010-03-30
  • 通讯作者: 刘建军

SET expression alteration in L-02 liver cells treated with trichloroethylene

JIANG Ying-zhi1;2;LIU Jian-jun2;ZHOU Gui-feng1;ZHANG Bing2;ZHENG Bo2;HUANG Ai-jun2   

  1. 1. Department of Preventive Medicine,The Medical College of Hunan Normal University, Changsha 410006, Hunan; 2. Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, Guangdong，China
  • Received:2009-10-30 Revised:2009-12-01 Online:2010-03-30 Published:2010-03-30
  • Contact: LIU Jian-jun

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摘要： 目的： 研究不同剂量三氯乙烯(TCE)对L-02肝细胞中SET mRNA和蛋白水平表达的影响，为探索TCE致肝损伤的机制提供实验依据。 方法： 分别用0.5％二甲基亚砜(DMSO)、3 mmol/L TCE及10 mmol/L TCE处理L-02肝细胞24 h后，采用实时荧光定量PCR(real-time quantitative PCR)技术和蛋白印迹(Western blotting)技术分别检测L-02肝细胞中SET mRNA和SET蛋白的表达。 结果： 与DMSO对照组相比，TCE处理组SET mRNA、 SET蛋白表达均显著上调(P均<0.01)，其10 mmol/L TCE处理组的SET蛋白表达高于3 mmol/L TCE处理组(P<0.05)，呈剂量依赖关系。 结论： TCE可诱导L-02肝细胞中SET表达上调，提示SET可能在TCE对肝细胞毒性效应中起着重要作用。

关键词: 三氯乙烯, SET, 蛋白印迹, 实时荧光定量PCR

Abstract: OBJECTIVE: To investigate the effects of different doses of trichloroethylene(TCE) on the expression alteration of SET gene at both the mRNA and protein levels, in order to provide some clues for the liver-injury mechanism of TCE. METHODS: Real-time quantitative PCR and Western blotting were employed to detect the mRNA and protein levels of SET in L-02 liver cells, which were separately treated with DMSO, 3 mmol/L and 10 mmol/L TCE. RESULTS: Compared with the control group, the mRNA and protein levels of SET were both significantly up-regulated (P<0.01) in the L-02 liver cells treated with TCE, and the expression of SET protein in the 10 mmol/L TCE group was higher than 3 mmol/L TCE group, showing a dose-dependent relationship. CONCLUSION: TCE could induce the expression of SET at both the mRNA and protein levels in a dose-dependent manner, which indicated that SET might play an important role in the hepatotoxicity of TCE.

Key words: trichloroethylene, SET, Western blotting, real-time quantitative PCR

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