重氮乙酰丝氨酸诱导大鼠胰腺腺泡细胞癌癌前病变及其K-ras 基因突变的实验研究

doi:10.3969/j.issn.1004-616x.1999.02.006

癌变·畸变·突变 ›› 1999, Vol. 11 ›› Issue (2): 80-085.doi: 10.3969/j.issn.1004-616x.1999.02.006

重氮乙酰丝氨酸诱导大鼠胰腺腺泡细胞癌癌前病变及其K-ras 基因突变的实验研究

徐　峰1 　曹　伟2 　寿楠海1 　姜希宏1 　徐克森1

1 山东医科大学附属医院普外科　2山东医科大学基础医学院药理学教研室　济南　250012

收稿日期:1998-01-17 修回日期:1998-06-16 出版日期:1999-03-30 发布日期:1999-03-30

THE EXPERIMENTAL STUDY OF PRECANCEROUS LESION OF PANCREATIC ACINAR CELLULAR CANCER IN RATS INDUCED BY AZASERINE AND THE DETECTION OF K2RAS MUTATION

Xu Feng1 , Cao Wei2 , Shou Nanhai1 ,J iang Xihong1 ,Xu Kesun1

1 Department of General S urgery , The A f f iliated Hospital of S handong Medical University , 2Department of Pharmacology , S handong Medical University 　Jinan

Received:1998-01-17 Revised:1998-06-16 Online:1999-03-30 Published:1999-03-30

摘要/Abstract

摘要： 建立大鼠胰腺腺泡细胞癌癌前病变的动物模型,了解该癌前病变及K2ras 基因突变的情况　雄性Wistar 大鼠单次腹腔注射重氮乙酰丝氨酸30mg/ Kg 体重,4 个月后常规取胰腺组织行光镜和电镜检查,并应用PCR2SSCP 法检测K2ras 基因突变情况　大鼠胰腺腺泡细胞癌癌前病变发生率100 % ,K2ras 基因突变率4211 %。重氮乙酰丝氨酸可成功诱导大鼠胰腺腺泡细胞癌癌前病变,K2ras 基因突变在此过程中属早期事件。

关键词: 重氮乙酰丝氨酸, 胰腺肿瘤, K-ras 基因

Abstract: To set up the animal model of precancerous lesion of rat’s pancreatic acinar cellular cancer , learn its biological feature and K2ras gene code 12 mutation. Male Wistar rat s were injected Azaserine 30mg/ kg (ip) . 4 months later , rat s’pancreas were autopsied and K2ras gene code 12 mutation were detected by PCR2SSCP. The incidence of precancreous lesion of rat s’pancreatic acinar cellular cancer was 42. 1 %. Azaserine could induce precancerous lesion of rat s’pancreatic acinar cancer successfully , K2ras gene code 12 mutation was early change during this process.

Key words: Azaserine, Pancreatic neoplasm, K-ras gene

徐　峰　曹　伟　寿楠海　姜希宏　徐克森. 重氮乙酰丝氨酸诱导大鼠胰腺腺泡细胞癌癌前病变及其K-ras 基因突变的实验研究[J]. 癌变·畸变·突变, 1999, 11(2): 80-085.

Xu Feng , Cao Wei , Shou Nanhai , J iang Xihong , Xu Kesun. THE EXPERIMENTAL STUDY OF PRECANCEROUS LESION OF PANCREATIC ACINAR CELLULAR CANCER IN RATS INDUCED BY AZASERINE AND THE DETECTION OF K2RAS MUTATION[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1999, 11(2): 80-085.

重氮乙酰丝氨酸诱导大鼠胰腺腺泡细胞癌癌前病变及其K-ras 基因突变的实验研究

THE EXPERIMENTAL STUDY OF PRECANCEROUS LESION OF PANCREATIC ACINAR CELLULAR CANCER IN RATS INDUCED BY AZASERINE AND THE DETECTION OF K2RAS MUTATION

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