关键词: 苯并[a]芘, 滴滴涕, 联合暴露, 谷丙转氨酶, 谷草转氨酶, γ-谷胺酰转移酶, 交互作用

Abstract: OBJECTIVE: To explore the effects of benzo[a] pyrene and DDT exposure alone or in combination on ALT,AST and γ-GT in mice. METHODS: Fifty healthy male Kunming mice were divided into 10 groups at random: blank control group (normal feed);solvent control group(equal volume of oil);1,10 mg/(kg·d) B[a]P groups;0.6,6 mg/(kg·d) DDT groups;1 mg/(kg·d) B[a]P+0.6 mg/(kg·d) DDT group;1 mg/(kg·d) B[a]P+6 mg/(kg·d) DDT group;10 mg/(kg·d) B[a]P+0.6 mg/(kg·d) DDT group and 10 mg/(kg·d) B[a]P+6 mg/(kg·d) DDT group. Exposure groups were treated with oil contained B[a]P and DDT once a day. After 31 days of intraperitoneal injection,eyeballs were extracted and ALT,AST and γ-GT were examined in blood. HE slices were made to confirm the liver damage. Using two factors and three levels factorial ANOVA design for data analysis. RESULTS: The levels of ALT and AST in all B[a]P or DDT alone exposure groups were significantly higher than that of the control groups (F=41.308,P=0.000;F=20.083,P=0.000),showing dose-effect relationships in both. But no interaction was observed in co-exposure groups(P=0.258,P=0.264). No significant changes in γ-GT were observed in all B[a]P or DDT alone exposure groups. Neither was there any interactive effect in co-exposure groups(P=0.816). This was in accordance with the observation in HE slice: hydropic degeneration,endolysis and nuclear enlargement in liver cell. CONCLUSION: Under this experimental condition,increasing levels of ALT and AST could be detected in B[a]P or DDT separate exposure groups. But neither could affect γ-GT level. No interactive effects of ALT,AST and γ-GT could be induced in co-exposure groups.

Key words: benzo[a]pyrene, DDT, co-exposure, ALT, AST, γ-GT, interactive effect