转人TNFα基因对肾细胞癌细胞致瘤性的影响

doi:10.3969/j.issn.1004-616x.2001.02.004

癌变·畸变·突变 ›› 2001, Vol. 13 ›› Issue (2): 77-080.doi: 10.3969/j.issn.1004-616x.2001.02.004

转人TNFα基因对肾细胞癌细胞致瘤性的影响

张晓琴1 , 陈　坚2 , 曹广文3 , 万谟彬1 , 戚中田3

1. 第二军医大学长海医院传染科, 上海　200433; 2. 第二军医大学军队卫生教研室, 上海　200433; 3. 第二军医大学微生物教研室, 上海　200433

收稿日期:2000-08-29 修回日期:2000-10-31 出版日期:2001-04-30 发布日期:2001-04-30

INFLUENCE ON THE TUMORIGENICITY OF THE HUMAN RENAL CELL CARCINOMA ( RCC) CELLS TRANSDUCED WITH HUMAN TNFα GENE

ZHANG Xiao-qin1, CHEN Jian2, CAO Guang-wen3, WAN Mo-bing1, QI Zhong-tian3.

1. Infection Department, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. Department of Military Hygiene, Second Military Medical University, Shanghai 200433, China; 3. Department of Microbiology, Second Military Medical University, Shanghai 200433, China

Received:2000-08-29 Revised:2000-10-31 Online:2001-04-30 Published:2001-04-30

摘要/Abstract

摘要： 目的:观察转人TNFα基因对肾细胞癌细胞(RCC) 致瘤性的影响,为进一步对RCC 的基因治疗打下基础。方法:将人TNFα基因构建入逆转录病毒载体,经包装、鉴定后,感染RCC 细胞株78620 , EL ISA 法测转人TNFα基因78620 细胞上清中TNFα的活性,同时,将转人TNFα基因的78620 细胞接种裸鼠,观测转TNFα前后RCC 细胞致瘤性的变化。结果: 转人TNFα78620 细胞上清TNFα的浓度平均为(5 004 ±624) pg/ ml ,接种裸鼠后无肿瘤长出。结论:转人TNFα基因使建株RCC 失去了致瘤性。

关键词: TNFα, 肾细胞癌, 致瘤性

Abstract: Purpose : To study the influence on the tumorigenicity of the human renal cell carcinoma (RCC) cells by t ransfection of human TNFαgene. Methods : hTNFαcDNA was inserted into ret roviral vector to const ruct recombinant vector pL ( TNFα) SN. The packaged and confirmed ret rovirus were infected to RCC cell line.Then , nude mice were injected by 78620 cell infected with the hTNFαgene or not , and the tumorigenecity of RCC was observed. Results : The mean expression level of TNFαin the supernatant of TNFα2t ransfected 78620 cell2culture medium was (5 004 ±624) pg/ ml. And the nude mice injected with TNFα gene engineered RCC cells had no tumor growth. Conclusion : The TNFαgene engineered human RCC cells lost tumorigenicity.

Key words: TNFα, renal cell carcinoma, tumorigenicity

中图分类号:

张晓琴, 陈　坚, 曹广文, 万谟彬, 戚中田. 转人TNFα基因对肾细胞癌细胞致瘤性的影响[J]. 癌变·畸变·突变, 2001, 13(2): 77-080.

ZHANG Xiao-qin, CHEN Jian, CAO Guang-wen, WAN Mo-bing, QI Zhong-tian.. INFLUENCE ON THE TUMORIGENICITY OF THE HUMAN RENAL CELL CARCINOMA ( RCC) CELLS TRANSDUCED WITH HUMAN TNFα GENE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2001, 13(2): 77-080.

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转人TNFα基因对肾细胞癌细胞致瘤性的影响

INFLUENCE ON THE TUMORIGENICITY OF THE HUMAN RENAL CELL CARCINOMA ( RCC) CELLS TRANSDUCED WITH HUMAN TNFα GENE

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