转cry1Ab/Ac基因大米对食蟹猴小肠黏膜形态及上皮内淋巴细胞和杯状细胞数量的影响

doi:10.3969/j.issn.1004-616x.2016.06.008

癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (6): 453-457,463.doi: 10.3969/j.issn.1004-616x.2016.06.008

转cry1Ab/Ac基因大米对食蟹猴小肠黏膜形态及上皮内淋巴细胞和杯状细胞数量的影响

杨艳伟, 周晓冰, 吕建军, 霍桂桃, 屈哲, 林志, 张頔, 王雪

中国食品药品检定研究院国家药物安全评价监测中心, 药物非临床安全评价研究北京市重点实验室, 北京 100176

收稿日期:2016-08-28 修回日期:2016-10-28 出版日期:2016-11-30 发布日期:2016-11-30
通讯作者: 王雪E-mail:xue_wang@nifdc.org.cn E-mail:xue_wang@nifdc.org.cn
作者简介:杨艳伟,E-mail:yangyanwei@nifdc.org.cn。
基金资助:
转基因生物新品种培育国家科技重大专项（2012ZX08011001）

Consumption of transgenic rice with the cry1Ab/Ac gene on intestinal mucosa, intraepithelial lymphocytes and goblet cells in Cynomolgus monkey

YANG Yanwei, ZHOU Xiaobing, LÜ Jianjun, HUO Guitao, QU Zhe, LIN Zhi, ZHANG Di, WANG Xue

Key Laboratory of Beijing for Nonclinical Safety Evaluation Research of Drugs, National Center for Safety Evaluation of Drugs, National Institutes for Food and Drug Control, Beijing 100176, China

Received:2016-08-28 Revised:2016-10-28 Online:2016-11-30 Published:2016-11-30

摘要/Abstract

摘要： 目的：研究转cry1Ab/Ac基因大米对食蟹猴小肠黏膜形态及上皮内淋巴细胞和杯状细胞数量的影响。方法：以转cry1Ab/Ac基因大米为受试物，选用24只食蟹猴，雌雄各半，随机分成3组，即普通大米对照组、转cry1Ab/Ac基因大米低、高剂量组。对照组、低剂量和高剂量组给予的猴饲料中转cry1Ab/Ac基因大米含量分别为0、17.5%和70%。每天饲喂2次，每次（100±10）g，持续6个月，恢复期4周。分别进行一般毒性指标的检测：临床症状观察、体质量、体温、心电图、血液学、血清生化、尿生化、脏器质量以及组织病理学检查。解剖取十二指肠、空肠、回肠进行HE染色和PAS染色，观察小肠黏膜形态、上皮内淋巴细胞及杯状细胞数目。结果：饲喂6个月后，与普通大米对照组相比，转cry1Ab/Ac基因大米低剂量组和高剂量组食蟹猴的一般常规检查未见异常。小肠黏膜形态各组均未见病理改变，低、高剂量组的上皮内淋巴细胞和杯状细胞数与对照组相比差异均无统计学意义（P>0.05）。结论：饲喂转cry1Ab/Ac基因大米6个月对食蟹猴小肠黏膜形态、上皮内淋巴细胞及杯状细胞数量未见不良影响。

关键词: cry1Ab/Ac基因, 上皮内淋巴细胞, 杯状细胞, 食蟹猴

Abstract: OBJECTIVE: To investigate the effect of transgenic rice with the cry1Ab/Ac gene on morphology of intestinal mucosa,cell number of intraepithelial lymphocytes and goblet cells in Cynomolgus monkey. METHODS: Twenty-four cynomolgus monkeys,half male and female,were randomly divided into 3 groups including control group of ordinary rice,low dose group and high dose groups of transgenic rice with the cry1Ab/Ac gene. Monkeys were fed twice a day and each feeding was (100±10) g for 6 months with 4 weeks recovery period. Monkeys were observed for general toxicity,clinical effects,body weight,body temperature,electrocardiogram,hematology,serum biochemistry, urinary biochemistry,organ weight and histopathological examination. HE and PAS staining were done for duodenum, jejunum and lleum. The morphology of intestinal mucosa,cell number of intraepithelial lymphocytes and goblet cells were observed under microscope. RESULTS: After six months,detection of transgenic rice with the cry1Ab/Ac gene were 0, 17.5% and 70%,in control,low dose and high dose groups,respectively. Compared with the control group,no abnormalities in toxicity,etc. were found in the treated groups. No pathological changes were observed in intestinal morphology. Cell number of intraepithelial lymphocytes and goblet cells were not statistically different between control and treated groups. CONCLUSION: Transgenic rice with the cry1Ab/Ac gene had no side effects on small intestine mucosal morphology,cell number of intraepithelial lymphocytes and goblet cells in Cynomolgus monkey.

Key words: cry1Ab/Ac gene, intraepithelial lymphocytes, goblet cells, Cynomolgus monkey

中图分类号:

R994.4

杨艳伟, 周晓冰, 吕建军, 霍桂桃, 屈哲, 林志, 张頔, 王雪. 转cry1Ab/Ac基因大米对食蟹猴小肠黏膜形态及上皮内淋巴细胞和杯状细胞数量的影响[J]. 癌变·畸变·突变, 2016, 28(6): 453-457,463.

YANG Yanwei, ZHOU Xiaobing, LÜ Jianjun, HUO Guitao, QU Zhe, LIN Zhi, ZHANG Di, WANG Xue. Consumption of transgenic rice with the cry1Ab/Ac gene on intestinal mucosa, intraepithelial lymphocytes and goblet cells in Cynomolgus monkey[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(6): 453-457,463.

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转cry1Ab/Ac基因大米对食蟹猴小肠黏膜形态及上皮内淋巴细胞和杯状细胞数量的影响

Consumption of transgenic rice with the cry1Ab/Ac gene on intestinal mucosa, intraepithelial lymphocytes and goblet cells in Cynomolgus monkey

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