长链非编码RNA XIST和miR-101-3p在子宫颈癌组织中的表达及关系

doi:10.3969/j.issn.1004-616x.2017.02.009

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (2): 121-124.doi: 10.3969/j.issn.1004-616x.2017.02.009

长链非编码RNA XIST和miR-101-3p在子宫颈癌组织中的表达及关系

刘平

南阳市第二人民医院妇产科, 河南南阳 473012

收稿日期:2016-12-07 修回日期:2017-02-24 出版日期:2017-03-31 发布日期:2017-03-31
作者简介:刘平,E-mail:m18538827625@163.com

Expression of LncRNA XIST and miR-101-3p in cervical cancer

LIU Ping

The Second People's Hospital of Nanyang, Nanyang 473012, Henan, China

Received:2016-12-07 Revised:2017-02-24 Online:2017-03-31 Published:2017-03-31

摘要/Abstract

摘要：

目的：探讨长链非编码RNA（LncRNA）XIST和miR-101-3p在子宫颈癌组织中的表达情况及其临床意义。方法：收集58例子宫颈癌和配对的癌旁组织标本，采用实时荧光定量PCR（qPCR）检测组织中LncRNA XIST和miR-101-3p的表达，分析其与子宫颈癌临床病理因素的相关性，并评价其临床意义。结果：与癌旁组织比较，qPCR显示宫颈癌组织中LncRNA XIST的表达明显上调（P < 0.05），miR-101-3p的表达明显下调（P < 0.05）；且二者在表达水平上呈负相关（r=-0.68，P=0.000）。LncRNA XIST和miR-101-3p的表达均与宫颈癌淋巴结转移、组织大小及临床分期之间存在相关性（P均<0.05）。结论：LncRNA XIST与miR-101-3p的表达呈负相关，可能成为早期筛选宫颈癌的生物标志物和治疗靶点。

关键词: 宫颈癌, LncRNA XIST, miR-101-3p, 淋巴结转移

Abstract:

OBJECTIVE: To investigate the expression and clinical significance of long non-coding RNA (LncRNA) XIST and miR-101-3p in cervical cancer. METHODS: Cervical cancer tissues and matched non-tumor para-neoplastic tissues were collected from 58 patients. Quantitative real-time PCR was used to examine the expression of LncRNA XIST and miR-101-3p,the correlation between them and their correlation with clinicopathological features were analyzed. RESULTS: LncRNA XIST expression was significantly up-regulated in cervical cancer tissues than in adjacent non-tumor tissues (P < 0.05). The expression of miR-101-3p was significantly down-regulated in cervical cancer tissues as compared with the adjacent tissues (P < 0.05).The expression of them in nucleic acid levels were negatively correlated(r=-0.68,P=0.000) and correlated significantly with FIGO stage,lymph node metastasis,and tissue size of tumor (P < 0.05). CONCLUSION: Our findings indicate that LncRNA XIST and miR-101-3p may be potential prognostic markers and therapeutic targets for cervical cancer. The expression of LncRNA XIST and miR-101-3p was negatively correlated.

Key words: cervical cancer, LncRNA XIST, miR-101-3p, lymphnode metastasis

中图分类号:

R737.33

刘平. 长链非编码RNA XIST和miR-101-3p在子宫颈癌组织中的表达及关系[J]. 癌变·畸变·突变, 2017, 29(2): 121-124.

LIU Ping. Expression of LncRNA XIST and miR-101-3p in cervical cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 121-124.

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长链非编码RNA XIST和miR-101-3p在子宫颈癌组织中的表达及关系

Expression of LncRNA XIST and miR-101-3p in cervical cancer

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