阿胶对人工细颗粒物所致大鼠呼吸系统损伤的保护作用

doi:10.3969/j.issn.1004-616x.2017.05.005

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (5): 346-351.doi: 10.3969/j.issn.1004-616x.2017.05.005

阿胶对人工细颗粒物所致大鼠呼吸系统损伤的保护作用

张飘飘¹, 凌亚豪¹, 阎晓丹¹, 魏金锋^1,2, 靳洪涛^1,2, 王爱平^1,2

1. 中国医学科学院北京协和医学院新药安全评价研究中心, 北京 100050;
2. 北京协和建昊医药技术开发有限责任公司, 北京 100176

收稿日期:2017-04-28 修回日期:2017-09-18 出版日期:2017-09-30 发布日期:2017-09-30
通讯作者: 靳洪涛,E-mail:jinhongtao@imm.ac.cn;王爱平,E-mail:wangaiping@imm.ac.cn E-mail:jinhongtao@imm.ac.cn;wangaiping@imm.ac.cn
作者简介:张飘飘,E-mail:purepiao@imm.ac.cn。
基金资助:
重大新药创制专项（2012ZX09301002-001-009）

Protective effects of Colla Corii Asini on artificial fine particulate matter-induced respiratory system injury in rats

ZHANG Piaopiao¹, LING Yahao¹, YAN Xiaodan¹, WEI Jinfeng^1,2, JIN Hongtao^1,2, WANG Aiping^1,2

1. New Drug Safety Evaluation Center, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100050;
2. Beijing Union-Genius Pharmaceutical Technology Development Co., Ltd., Beijing 100176, China

Received:2017-04-28 Revised:2017-09-18 Online:2017-09-30 Published:2017-09-30

摘要/Abstract

摘要： 目的：探讨阿胶对人工细颗粒物（aPM2.5）所致大鼠呼吸系统损伤的保护作用及其机制。方法：SD大鼠96只，雌雄各半，随机分为对照组、模型组、阿胶低剂量组（0.3 g/kg）、阿胶高剂量组（1.2 g/kg）。模型组和阿胶组大鼠口鼻吸入（3.68±0.41） mg/L的aPM2.5，每天1次，每次15 min，连续8周，对照组吸入清洁空气。阿胶组于吸入aPM2.5前1 h灌胃给予阿胶，模型组和对照组灌胃饮用水。试验期间每两周采用EMKA动物肺功能监测系统测定大鼠肺功能，分别于染毒后第2、4、8周各取8只大鼠，采血并收集血清、肺泡灌洗液和肺组织，检测血清和肺泡灌洗液氧化损伤相关指标的变化，进行肺泡灌洗液白细胞计数及肺脏组织病理学检查。结果：与对照组相比，模型组大鼠潮气流量（TV）、呼气量（EV）、分时输出量（MV）等容量指标均显著降低（P均 < 0.05），吸气时间（Ti）、呼气时间（Te）、弛豫时间（RT）等时间指标显著均升高（P均 < 0.05）；与模型组相比，阿胶可显著抑制aPM2.5所致大鼠TV、EV、MV的降低及Ti、Te、RT的升高（P < 0.05）。染毒后第8周，模型组与对照组比较大鼠肺巨噬细胞增多，阿胶高剂量组肺巨噬细胞较模型组轻度降低。与对照组相比，模型组大鼠血清和肺泡灌洗液（BALF）中丙二醛（MDA）含量升高，而阿胶可抑制MDA含量的增加。与对照组相比，模型组大鼠的血清和BALF中谷胱甘肽过氧化物酶（GSH-Px）活性有所降低，阿胶可升高GSH-Px的活性。结论：阿胶可改善aPM2.5造成的大鼠呼吸功能降低，抑制肺巨噬细胞的增多，该作用可能与其抑制aPM2.5所致氧化损伤有关，确切机制需要进一步深入研究。

关键词: 人工细颗粒物, 阿胶, 呼吸功能, 氧化损伤

Abstract: OBJECTIVE: To study the protective effects of Colla Corii Asini on respiratory system injury which was induced by artificial fine particulate matter (aPM2.5) in rats. METHODS: 96 SD rats(half male and half female)were randomly divided into four groups, control,model,Colla Corii Asini low dose (0.3 g/kg) and high dose (1.2 g/kg) groups. Rats in both model and Colla Corii Asini groups were exposed to aPM2.5 15 minutes per day of 7 days a week for 8 weeks using the aerosol exposure system. In contrast,rats in the control group were exposed to clear air. 1 h before daily exposure to aPM2.5,rats of Colla Corii Asini groups were given Colla Corii Asini by intragastric administration. During the experimental period,the EMKA animal lung function monitoring system was used to monitor the lung function of rats. Every two weeks,blood serum and bronchoalveolar lavage fluid (BALF),and lung tissues were collected to detect changes in biomarkers of oxidative injury. RESULTS: Compared with the control group,the tidal volume (TV),expiratory volume (EV) and minute volume (MV) of the model group were markedly reduced,while the inspiratory time (Ti),expiratory time (Te) and relaxation time (RT) were significantly increased.,However,treating rats with Colla Corii Asini before aPM2.5 exposure significantly prevented the decline of TV,MV,EV and the increase of Ti,RT,Te. The lung phagocyte counts of the model group increased significantly compared with the control group,while the situation of Colla Corii Asini high dose group achieved a certain degree of relief. Compared with the control group,the concentration of methane dicarboxylic aldehyde (MDA) in serum and BALF of the model group increased,and with the treatment of Colla Corii Asini,the MDA decreased. Compared with the control group,the acvtivity of glutathione peroxidase (GSH-Px) in serum and BALF of the model group decreased,and with the treatment of Colla Corii Asini,the the acvtivity of glutathione peroxidase (GSH-Px) increased. CONCLUSION: Treatment of the model rats with Colla Corii Asini prevented decline of lung function and reduced lung phagocyte counts which were caused by aPM2.5,It is possible that Colla Corii Asini can prevent oxidative damage caused by aPM2.5. Further study is needed to address the mechanism for interactions.

Key words: artificial fine particulate matter, Colla Corii Asini, respiratory function, oxidative damage

中图分类号:

R994.6

张飘飘, 凌亚豪, 阎晓丹, 魏金锋, 靳洪涛, 王爱平. 阿胶对人工细颗粒物所致大鼠呼吸系统损伤的保护作用[J]. 癌变·畸变·突变, 2017, 29(5): 346-351.

ZHANG Piaopiao, LING Yahao, YAN Xiaodan, WEI Jinfeng, JIN Hongtao, WANG Aiping. Protective effects of Colla Corii Asini on artificial fine particulate matter-induced respiratory system injury in rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 346-351.

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阿胶对人工细颗粒物所致大鼠呼吸系统损伤的保护作用

Protective effects of Colla Corii Asini on artificial fine particulate matter-induced respiratory system injury in rats

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