PM2.5对斑马鱼胚胎发育早期氧化应激和DNA甲基化调控相关基因表达的影响

doi:10.3969/j.issn.1004-616x.2017.05.011

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (5): 379-382.doi: 10.3969/j.issn.1004-616x.2017.05.011

PM2.5对斑马鱼胚胎发育早期氧化应激和DNA甲基化调控相关基因表达的影响

吴德生, 杨飞, 袁建辉, 杨淋清, 黄海燕, 刘建军, 庄志雄

深圳市疾病预防控制中心深圳市现代毒理学重点实验室, 广东深圳 518055

收稿日期:2017-04-25 修回日期:2017-06-28 出版日期:2017-09-30 发布日期:2017-09-30
通讯作者: 袁建辉,E-mail:jianhui_yuan@126.com E-mail:jianhui_yuan@126.com
作者简介:吴德生,E-mail:dswucn@126.com。
基金资助:
深圳市科技研发资金资助项目（JCY20140410171018510，JCYJ20 140410164217365，JCYJ20 160328144536436）；深圳市卫计委重点学科建设能力提升项目（201506064）

Effect of PM2.5 on expression of oxidative stress and DNA methylation genes during early development of zebrafish embryos

WU Desheng, YANG Fei, YUAN Jianhui, YANG Linqing, HUANG Haiyan, LIU Jianjun, ZHUANG Zhixiong

Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China

Received:2017-04-25 Revised:2017-06-28 Online:2017-09-30 Published:2017-09-30

摘要/Abstract

摘要： 目的：探讨细颗粒物（PM2.5）暴露后，斑马鱼胚胎发育过程中氧化应激和DNA甲基化基因表达的改变。方法：使用大功率大气PM2.5采样器收集PM2.5样品，超声后冷冻提取PM2.5，分别以0、5、20 μg/mL的浓度作用于斑马鱼胚胎。在暴露后2、6、24、48 h提取胚胎RNA，用荧光定量PCR法检测氧化应激相关基因超氧化物歧化酶1（sod1）和8-羟基鸟嘌呤DNA糖苷酶（ogg1）以及DNA甲基化相关基因DNA羟化酶1（tet1）、DNA甲基转移酶（dnmt1） mRNA的表达。结果：斑马鱼胚胎暴露于0、5、20 μg/mL的PM2.5后2~6 h，sod1、tet1和dnmt1的mRNA相对表达量均随着剂量升高而明显升高，呈剂量-效应关系（r依次=0.98、0.98、0.99，P均 < 0.05）；PM2.5暴露后2~6 h ogg1的mRNA相对表达量在5 μg/mL剂量组显著升高（P < 0.05）。而PM2.5暴露后24~48 h，sod1、ogg1、tet1和dnmt1的mRNA相对表达量相对2~6 h均明显下降（P < 0.05）。结论：PM2.5对斑马鱼胚胎的氧化应激相关基因sod1和ogg1以及DNA甲基化相关基因tet1和dnmt1的mRNA表达均有影响。

关键词: 细颗粒物, 胚胎发育, 氧化应激, DNA甲基化

Abstract: OBJECTIVE: To explore effects of PM2.5 exposure on expression of oxidative stress and DNA methylation genes during zebrafish embryo development. METHODS: Samples of PM2.5 were collected by large-volume samplers on fiber filters. Then,the particle materials were collected by ultrasonicing and freeze-drying. Zebrafish embryos were exposed to PM2.5 particle materials in the concentrations of 0,5 and 20 μg/mL. After extraction of total RNA,relative expression of oxidative stress genes (sod1 and ogg1) and DNA methylation genes (tet1 and dnmt1) was detected by qPCR. RESULTS: 2-6 h after PM2.5 exposure,expression of sod1,tet1 and dnmt1 were increased significantly with a dose-dependent relationship (r=0.98,0.98,0.99,P < 0.05,respectively);2-6 h after PM2.5 exposure,expression of ogg1 was increased significantly in the 5 μg/mL group (P < 0.05);24-48 h after PM2.5 exposure,expression of sod1,ogg1,tet1 and dnmt1 were decreased significantly compared with those in early stage (2-6 h). CONCLUSION: Expression of oxidative stress genes (sod1 and ogg1) and DNA methylation genes (tet1 and dnmt1) could be affected from exposure to PM2.5,especially during early embryo development.

Key words: PM2.5, embryo development, oxidative stress, DNA methylation

中图分类号:

R994.6

吴德生, 杨飞, 袁建辉, 杨淋清, 黄海燕, 刘建军, 庄志雄. PM2.5对斑马鱼胚胎发育早期氧化应激和DNA甲基化调控相关基因表达的影响[J]. 癌变·畸变·突变, 2017, 29(5): 379-382.

WU Desheng, YANG Fei, YUAN Jianhui, YANG Linqing, HUANG Haiyan, LIU Jianjun, ZHUANG Zhixiong. Effect of PM2.5 on expression of oxidative stress and DNA methylation genes during early development of zebrafish embryos[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 379-382.

参考文献

[1] SHIRAIWA M,LI Y,TSIMPIDI A P,et al. Global distribution of particle phase state in atmospheric secondary organic aerosols[J]. Nat Commun,2017,8:15002.
[2] SUN J,ZHOU T. Health risk assessment of China's main air pollutants[J]. BMC Public Health,2017,17(1):212.
[3] LAWAL A O. Air particulate matter induced oxidative stress and inflammation in cardiovascular disease and atherosclerosis:The role of Nrf2 and AhR-mediated pathways[J]. Toxicol Lett,2017,270(1):88-95.
[4] KANWAL M,DING X J,CAO Y. Familial risk for lung cancer[J]. Oncol Lett,2017,13(2):535-542.
[5] FUKS K B,WEINMAYR G,BASAGAÑA X,et al. Long-term exposure to ambient air pollution and traffic noise and incident hypertension in seven cohorts of the European study of cohorts for air pollution effects (ESCAPE)[J]. Eur Heart J,2017,38(13):983-990.
[6] LAURENT O,HU J,LI L,et al. Low birth weight and air pollution in California:Which sources and components drive the risk?[J]. Environ Int,2016,92/93(4):471-477.
[7] VERAS M M,DE OLIVEIRA ALVES N,FAJERSZTAJN L,et al. Before the first breath:prenatal exposures to air pollution and lung development[J]. Cell Tissue Res,2017,367(3):445-455.
[8] STAPLETON P A. Gestational nanomaterial exposures:microvascular implications during pregnancy,fetal development and adulthood[J]. J Physiol,2016,594(8):2161-2173.
[9] YU Y,WANG X,WANG B,et al. Polycyclic aromatic hydrocarbon residues in human milk,placenta,and umbilical cord blood in Beijing,China[J]. Environ Sci Technol,2011,45(23):10235-10242.
[10] SAENEN N D,VRIJENS K,JANSSEN B G,et al. Placental nitrosative stress and exposure to ambient air pollution during gestation:A population study[J]. Am J Epidemiol,2016,184(6):442-449.
[11] FUKUI H,ENDOH S,SHICHIRI M,et al. The induction of lipid peroxidation during the acute oxidative stress response induced by intratracheal instillation of fine crystalline silica particles in rats[J]. Toxicol Ind Health,2016,32(8):1430-1437.
[12] ZHANG J,LIU L,REN L,et al. The single and joint toxicity effects of chlorpyrifos and beta-cypermethrin in zebrafish (Danio rerio) early life stages[J]. J Hazard Mater,2017,334(5):121-131.
[13] CUNHA V,RODRIGUES P,SANTOS M M,et al. Danio rerio embryos on Prozac-effects on the detoxification mechanism and embryo development[J]. Aquat Toxicol,2016,178(8):182-189.
[14] DING R,JIN Y,LIU X,et al. Characteristics of DNA methylation changes induced by traffic-related air pollution[J]. Mutat Res Genet Toxicol Environ Mutagen,2016,796(1):46-53.
[15] ROSSNEROVA A1,TULUPOVA E,TABASHIDZE N. Factors affecting the 27K DNA methylation pattern in asthmatic and healthy children from locations with various environments[J]. Mutat Res,2013,741/742(1):18-26.

PM2.5对斑马鱼胚胎发育早期氧化应激和DNA甲基化调控相关基因表达的影响

Effect of PM2.5 on expression of oxidative stress and DNA methylation genes during early development of zebrafish embryos

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	艾多, 徐安琦, 孔德钦, 刘颖, 于卫华, 王钊, 彭洁, 刘瑞, 张晓迪, 海春旭, 李文丽, 王欣, 刘江正. 催化性抗氧化剂AEOL-10150对氮芥诱导小鼠急性肝损伤的保护作用[J]. 癌变·畸变·突变, 2022, 34(1): 40-46,61.
[2]	尹亚萍, 王宇, 田雨阳, 马焜, 李百祥. 褪黑素在帕金森病治疗中的研究进展[J]. 癌变·畸变·突变, 2022, 34(1): 75-78,81.
[3]	曹家豪, 彭越, 段佳琪, 刘桥松, 刘启玲, 秦绪军. 酒精性脂肪肝的线粒体作用机制研究进展[J]. 癌变·畸变·突变, 2021, 33(6): 475-481.
[4]	李柏茹, 秦双建, 关岚, 李闰冰, 蔡颖, 蒲桔宁, 曾明, 肖芳, 徐新云. PM_2.5对HK-2细胞氧化损伤和凋亡的影响[J]. 癌变·畸变·突变, 2021, 33(5): 360-364.
[5]	李宏伟, 孔德钦, 于卫华, 吴昊, 王钊, 刘瑞, 海春旭, 王欣, 刘江正, 李文丽. 齐墩果酸对酒精诱导的大鼠胃壁氧化损伤的保护作用[J]. 癌变·畸变·突变, 2021, 33(5): 365-369.
[6]	蒋杉, 杜凤, 康秉文, 殷草草, 周健, 石莹, 王玥, 周耿瑶, 秦绪军. 异鼠李素通过减轻氧化应激延缓D-半乳糖诱导的人脐静脉内皮细胞衰老[J]. 癌变·畸变·突变, 2021, 33(2): 95-100.
[7]	刘洁仪, 张睿, 夏冬, 李镕, 李晓雯, 陈姿旭, 魏青, 赵志强, 何云. PM_2.5中不同组分对人脐静脉内皮细胞的联合毒性作用研究[J]. 癌变·畸变·突变, 2021, 33(2): 116-123,128.
[8]	李柏茹, 秦双建, 李闰冰, 蔡颖, 郑凯, 曾明, 肖芳, 徐新云. p38MAPK基因对PM_2.5染毒HK-2细胞部分癌基因和凋亡相关基因表达的影响[J]. 癌变·畸变·突变, 2021, 33(2): 129-135,142.
[9]	李柏茹, 秦双建, 李闰冰, 蔡颖, 郑凯, 王冰玉, 曾明, 肖芳, 徐新云. PM_2.5对HK-2肾细胞部分癌基因和凋亡基因表达的影响[J]. 癌变·畸变·突变, 2021, 33(1): 6-11.
[10]	赵俊伟, 华辰凤, 尚平平, 谢复炜, 李翔. 亚砷酸As (Ⅲ)诱导人肺癌A549细胞氧化应激反应的实验研究[J]. 癌变·畸变·突变, 2021, 33(1): 28-31.
[11]	张力, 张爱华, 王文娟. 甲基汞暴露致学习记忆功能障碍作用机制的研究进展[J]. 癌变·畸变·突变, 2020, 32(6): 490-493.
[12]	蔡颖, 李闰冰, 郑凯, 秦双建, 李柏茹, 张朝晖, 徐新云. PM_2.5染毒HBE细胞前后差异表达的microRNAs及其生物信息学分析[J]. 癌变·畸变·突变, 2020, 32(5): 355-362.
[13]	秦双建, 李柏茹, 蔡颖, 郑凯, 王冰玉, 李闰冰, 肖芳, 曾明, 徐新云. PM_2.5对L02肝细胞部分癌基因和凋亡相关基因表达的影响[J]. 癌变·畸变·突变, 2020, 32(4): 281-285.
[14]	蔡颖, 郑凯, 秦双建, 李柏茹, 余淑苑, 刘宁, 季佳佳, 张朝晖, 徐新云. 沉默c-fos基因对PM_2.5染毒人支气管上皮细胞癌基因和凋亡相关基因表达的影响[J]. 癌变·畸变·突变, 2020, 32(4): 298-303,308.
[15]	孙娇娇, 车碧众, 罗秋林, 翟兵中, 信丽丽. 1,25-二羟基维生素D₃对PM_2.5所致HBE细胞氧化损伤的保护作用[J]. 癌变·畸变·突变, 2020, 32(2): 92-97.