异欧前胡素对人表皮黑素细胞酪氨酸酶和Rab27a的调节作用

doi:10.3969/j.issn.1004-616x.2018.01.007

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (1): 37-41.doi: 10.3969/j.issn.1004-616x.2018.01.007

异欧前胡素对人表皮黑素细胞酪氨酸酶和Rab27a的调节作用

孙贝蕾¹, 郑宇琼², 林玲¹, 陈玮莹¹, 蔡尔慧³, 陈玮芳³

1. 汕头大学医学院基础医学实验教学中心, 广东汕头 515041;
2. 汕头大学医学院第一附属医院, 广东汕头 515041;
3. 汕头大学医学院第二附属医院, 广东汕头 515041

收稿日期:2017-09-26 修回日期:2017-12-08 出版日期:2018-01-30 发布日期:2018-01-30
通讯作者: 蔡尔慧,E-mail:ehcai@stu.edu.cn;陈玮芳,E-mail:cwflxk2@139.com E-mail:ehcai@stu.edu.cn;cwflxk2@139.com
作者简介:孙贝蕾,E-mail:923719157@qq.com;郑宇琼,E-mail:hwl6999@21cn.com。
基金资助:
广东省中医药局项目（20142082）

Effects of isoimperaterin on regulation of tyrosinase and Rab27a in human epidermal melanocytes

SUN Beilei¹, ZHENG Yuqiong², LIN Ling¹, CHEN Weiying¹, CAI Erhui³, CHEN Weifang³

1. Experimental Teaching Center for Preclinical Medicine, Shantou University Medical College, Shantou 515041;
2. The First Affiliated Hospital, Shantou University Medical College, Shantou 515041;
3. The Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2017-09-26 Revised:2017-12-08 Online:2018-01-30 Published:2018-01-30

摘要/Abstract

摘要： 目的：探讨异欧前胡素对体外培养的人表皮黑素细胞酪氨酸酶和Rab27a的凋节作用。方法：体外分离人表皮黑素细胞并纯化培养，随机分为对照组和实验组。对照组加入M-254培养基，实验组加入含25 μmol/L异欧前胡素的M-254培养基，培养48 h后倒置显微镜下观察黑素细胞的形态；MTT法检测黑素细胞活力；Western blot法检测黑素细胞酪氨酸酶和转运相关蛋白Rab27a的表达；L-多巴氧化法和NaOH法测定黑素细胞酪氨酸酶活性和黑素含量。结果：与对照组比较，实验组人表皮黑素细胞的树状突起变长，黑素细胞活力降低17.2%（P < 0.05），黑素细胞酪氨酸酶表达下降30.3%（P < 0.01），而Rab27a表达升高31.9%（P < 0.05）；酪氨酸酶活性降低25.6%（P < 0.05）；黑素含量降低30.9%（P < 0.01）。结论：25 μmol/L异欧前胡素使黑素细胞的树状突起变长，抑制黑素细胞活力，抑制黑素细胞酪氨酸酶的表达和活性，促进黑素转运相关蛋白Rab27a的表达，降低黑素细胞中黑素的含量。

关键词: 黑素细胞, 异欧前胡素, 酪氨酸酶, Rab27a, 黑素

Abstract: OBJECTIVE: To investigate the effects of isoimperaterin on regulation of tyrosinase and Rab27a in cultured human epidermal melanocytes. METHODS: Human epidermal melanocytes were isolated and cultured. The cells were randomly divided into control and experimental groups. Melanocytes of the control group were cultured with M-254 culture medium. Cells of the experimental group were cultured with M-254 culture medium containing 25 μmol/L isoimperaterin. The cells were cultured for 48 h and observed using inverted microscopy. Cell vitality was assayed using MTT method. The expression of tyrosinase and transfer-related protein Rab27a were analyzed by western blotting. The activity of tyrosinase and the content of melanin were measured by L-dopa oxidation and NaOH method,respectively. RESULTS: Compared with the control group,the dendrites of melanocytes of experimental group were elongated after treatment with 25 μmol/L isoimperaterin for 48 h. Melanocyte vitality was reduced by 17.2% (P < 0.05). Expression of tyrosinase was decreased by 30.3% (P < 0.01) and Rab27a was increased by 31.9% (P < 0.05). The activity of tyrosinase was reduced by 25.6% (P < 0.05). Melanin content was decreased by 30.9% (P < 0.01). CONCLUSION: 25 μmol/L isoimperaterin elongated melanocytic dendrites but inhibited melanocyte vitality. It also inhibited the expression and activity of tyrosinase and promoted the expression of transfer-related protein Rab27a to reduce the content of melanin in cultured human epidermal melanocytes.

Key words: melanocyte, isoimperatorin, tyrosinase, Rab27a, melanin

中图分类号:

R329.2

孙贝蕾, 郑宇琼, 林玲, 陈玮莹, 蔡尔慧, 陈玮芳. 异欧前胡素对人表皮黑素细胞酪氨酸酶和Rab27a的调节作用[J]. 癌变·畸变·突变, 2018, 30(1): 37-41.

SUN Beilei, ZHENG Yuqiong, LIN Ling, CHEN Weiying, CAI Erhui, CHEN Weifang. Effects of isoimperaterin on regulation of tyrosinase and Rab27a in human epidermal melanocytes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 37-41.

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异欧前胡素对人表皮黑素细胞酪氨酸酶和Rab27a的调节作用

Effects of isoimperaterin on regulation of tyrosinase and Rab27a in human epidermal melanocytes

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