鹿血清预处理对大鼠心肌细胞缺糖损伤的保护作用

doi:10.3969/j.issn.1004-616x.2021.01.004

癌变·畸变·突变 ›› 2021, Vol. 33 ›› Issue (1): 17-21.doi: 10.3969/j.issn.1004-616x.2021.01.004

鹿血清预处理对大鼠心肌细胞缺糖损伤的保护作用

赵宁^1,3, 杜健鹏², 孙震晓³

1. 中国中医科学院西苑医院药剂科, 北京 100091;
2. 中国中医科学院西苑医院心血管科, 北京 100091;
3. 北京中医药大学生命科学学院, 北京 102488

收稿日期:2020-11-08 修回日期:2021-01-08 出版日期:2021-01-30 发布日期:2021-02-06
通讯作者: 孙震晓,E-mail:sunzxcn@hotmail.com E-mail:sunzxcn@hotmail.com
作者简介:赵宁,E-mail:zhaoning0606@126.com。
基金资助:
国家自然科学基金青年基金（81303126）

Protective activities against induced cardiomyocyte injury by deer serum

ZHAO Ning^1,3, DU Jianpeng², SUN Zhenxiao³

1. Department of Pharmacy, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091;
2. Department of Cardiology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091;
3. School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China

Received:2020-11-08 Revised:2021-01-08 Online:2021-01-30 Published:2021-02-06

摘要/Abstract

摘要： 目的： 研究鹿血清预处理对心肌细胞缺糖损伤的影响及作用机制。方法： 心肌细胞采用无糖DMEM培养基培养18 h造成缺糖损伤，模拟心肌缺血再灌注实验，观察鹿血清预处理6和12 h两种情况是否减轻缺糖损伤的心肌细胞，并选用羊血清作对照。按随机分组法分为正常对照组、模型组、鹿血清组（5、10和20 mg/mL）及羊血清组（5、10和20 mg/mL），观察各组细胞的形态学变化，检测心肌细胞相对活力，细胞培养液中乳酸脱氢酶（LDH）、丙二醛（MDA）浓度以及谷胱甘肽过氧化物酶（GSH-PX）和超氧化物歧化酶（SOD）活力等指标。结果： 预处理6 h情况下，与模型组、正常对照组相比，不同浓度鹿血清及羊血清均显著增加心肌细胞相对活力（P < 0.01），鹿血清与羊血清组间的差异无统计学意义（P > 0.05）。预处理12 h情况下，不同浓度（5、10、20 mg/mL）鹿血清组心肌细胞相对活力高于模型组（P < 0.01），但低于正常对照组（P < 0.05）；不同浓度（5、10、20 mg/mL）羊血清组心肌细胞相对活力与模型组间的差异无统计学意义（P > 0.05）；20 mg/mL鹿血清组心肌细胞相对活力高于20 mg/mL羊血清组（P < 0.01）；与模型组比较，不同浓度（5、10、20 mg/mL）鹿血清组细胞培养液中LDH浓度降低（P < 0.01），20 mg/mL鹿血清组及不同浓度（5、10、20 mg/mL）羊血清组GSH-PX活性升高（P < 0.01），10 mg/mL鹿血清组SOD活性升高（P < 0.01）。结论： 鹿血清可保护心肌细胞缺糖损伤，其机制可能与对抗氧自由基损伤及稳定细胞膜有关。

关键词: 鹿血清, 预处理, 心肌细胞, 缺糖

Abstract: OBJECTIVE: To investigate protective potential of deer serum on cardiomyocyte injury which was induced by glucose deprivation. METHODS: Cardiomyocytes were cultured in vitro and were subjected to glucose deprivation for 18 h to induce myocardial cell injury. An objective was to observe whether deer serum would alleviate the injury by 6 h and 12 h preconditioning, using sheep serum as comparison. The cardiomyocyte cultures were divided into several groups:normal control, model, deer serum-treated (5、10 and 20 mg/mL) and sheep serum-treated (5, 10 and 20 mg/mL) groups. Effects of cell morphology, relative activity of myocardial cells, lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) content, glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activity were used as evaluation indexes. RESULTS: Compared with the model and the normal control groups, the 6 h pretreatment by both sera significantly increased the relative activities of cardiomyocytes at different concentrations (P < 0.01), but there was no significant difference between the deer and the sheep serum groups (P > 0.05). After pretreatment for 12 h with different concentrations of deer serum, the relative activities of myocardial cells were significantly increased over the model group (P < 0.01), but the increases were lower than that of the normal control group (P < 0.05). There was no statistical significance in activities after treatment with the different concentrations of sheep serum (P > 0.05). The relative activities of myocardial cells in the 20 mg/mL deer serum group was significantly higher than that in the 20 mg/mL sheep serum group (P < 0.01).On the other hand, the concentrations of LDH in the deer serum groups was significantly decreased compared with the model group (P < 0.01). Activities of GSH-Px in the 20 mg/mL deer serum group and in different concentrations (5, 10, 20 mg/mL) of the sheep serum groups were significantly increased (P < 0.01), and activities of SOD in the 10 mg/mL deer serum group was significantly increased (P < 0.01). CONCLUSION: Deer serum preconditioning had a protective effect against cardiomyocyte apoptosis which was induced by glucose deprivation. The mechanism may be related to protection against oxygen free radical damage and stabilization of cell membrane.

Key words: deer serum, pretreatment, cardiomyocyte, no glucose

中图分类号:

R966

赵宁, 杜健鹏, 孙震晓. 鹿血清预处理对大鼠心肌细胞缺糖损伤的保护作用[J]. 癌变·畸变·突变, 2021, 33(1): 17-21.

ZHAO Ning, DU Jianpeng, SUN Zhenxiao. Protective activities against induced cardiomyocyte injury by deer serum[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(1): 17-21.

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鹿血清预处理对大鼠心肌细胞缺糖损伤的保护作用

Protective activities against induced cardiomyocyte injury by deer serum

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