癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (3): 171-174.doi: 10.3969/j.issn.1004-616x.2010.03.003

• 论著 • 上一篇    下一篇

胰岛素启动子在肝干细胞分化研究中的应用

金彩霞1;2;李文林1;李元杰2;徐方2;胡以平1   

  1. 1. 第二军医大学细胞生物学教研室，上海 200433； 2. 宁夏医科大学医学遗传学与细胞生物学教研室，宁夏 银川 750004
  • 收稿日期:2009-10-09 修回日期:2010-01-05 出版日期:2010-05-30 发布日期:2010-05-30
  • 通讯作者: 胡以平;徐方

Utilization of insulin promoter in differentiation of liver progenitor cells

JIN Cai-xia1;2;LI Wen-lin1;LI Yuan-jie2;XU Fang2;HU Yi-ping1   

  1. 1.Department of Cell Biology, College of Basic Medical Science, Second Military Medical University, Shanghai 200433; 2.Department of Medical Genetics and Cell Biology, Ningxia Medical University, Yinchuan 750004
  • Received:2009-10-09 Revised:2010-01-05 Online:2010-05-30 Published:2010-05-30
  • Contact: HU Yi-ping;XU Fang

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摘要： 目的： 利用报告基因监测肝干细胞向胰腺细胞的转分化潜能。 方法： 通过PCR从大鼠鼠尾基因组中克隆胰岛素启动子片段，构建大鼠胰岛素Ⅰ启动子调控的红色荧光蛋白报告载体(pRIP Ds Red)，并利用报告载体标记的肝原始细胞系(liver epithelial progenitor cells, LEPCs)及经逆转录病毒感染后表达MafA的肝原始细胞系(LEPCs-MafA)，观察肝干细胞向胰腺细胞的转分化潜能。 结果： 获得胰岛素启动子调控的报告载体、报告载体标记的LEPCs及LEPCs-MafA。 结论： pRIP DsRed报告载体的成功构建为研究LEPCs向胰腺细胞的转分化提供了简捷的分子工具。

关键词: 肝干细胞, 胰岛素启动子, 转分化, 红色荧光蛋白

Abstract: OBJECTIVE: To construct the report gene expression vector for studying the transdifferentiation potency of liver epithelial progenitor cells (LEPCs). METHODS: The rat insulin Ⅰ promoter(RIP) was amplified by PCR from rat tail genomic DNA and subcloned into promoter-removed pDsRed1-1 vector. By using the reporter gene tagging cells, the phenotype variation of LEPCs transdifferentiate into pancreas-like cells could be evaluated. RESULTS: RIP report gene expression vector could monitor the transdifferentiation of LEPCs into pancreas-like cells in a real-time manner. CONCLUSION: The reporter vector could useful for studying the transdifferentiation of LEPCs into pancreas-like cells.

Key words: liver epithelial progenitor cells, rat insulin promoter, transdifferentiation, red fluorescent protein

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