癌变·畸变·突变 ›› 2003, Vol. 15 ›› Issue (3): 183-185.doi: 10.3969/j.issn.1004-616x.2003.03.018

• 技术与方法 • 上一篇    下一篇

小鼠淋巴瘤细胞突变试验方法

谢　明   

  1. 化学工业农药安全评价质量监督检验中心暨国家新药安全评价研究中心，辽宁　沈阳　110021
  • 收稿日期:2002-04-13 修回日期:2002-11-25 出版日期:2003-07-30 发布日期:2003-07-30
  • 通讯作者: 谢　明

THE MOUSE LYMPHOMA CELL MUTATION ASSAY

XIE Ming   

  1. Supervision and Test Center f or Pesticide Safety Evaluation and Quality Control ＆ Nation Research Center for New Drug Safty Evalution,Shenyang 110021,China
  • Received:2002-04-13 Revised:2002-11-25 Online:2003-07-30 Published:2003-07-30
  • Contact: XIE Ming

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摘要： 小鼠淋巴瘤细胞突变试验(简称小鼠淋巴瘤试验或MLA)是国际上较常应用的遗传毒性试验常规方法，有关该试验方面的报道在国内不常见，本文对该试验方法作了较为具体介绍，仅围绕该试验的96孔板法(microwell or microtitre)展开论述，具有较好的可操作性。

关键词: 小鼠淋巴瘤细胞试验, 致突变试验, 96孔板法

Abstract: The mouse lymphoma cell mutation assay (shortened as the mouse lymphoma assay or MLA) is often used as a general genetic toxicological assay abroad in order to evaluate the genotoxicity risk. But MLA isn't much often reported in China. In this paper, the assay method and current protocol of MLA is not discussed and described. The areas discussed for MLA were only be on a typical microwell protocol. The method and procedure described were available and could be handled better according to the discription.

Key words: mouse lymph oma assay, mutagenicity test, 96－well microwell method

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