关键词: 二甲氨基偶氮苯, 肝癌, CYP2D1基因, CYP2D2基因, CYP2D4基因, 突变

Abstract: BACKGROUND ＆ AIM： To analyze the expressions and their sequences of cytochrome P4502D(CYP2D) subfamily gene in rat heptocarcinoma induced by dimethylaminoazobenzene(DAB). MATERIAL AND METHODS： The rats were induced by azo dye DAB ,the mRNA expression of three isoforms(CYP2D1、CYP2D2 and CYP2D4)were detected by quantitative RT-PCR,then PCR products were sequenced and analyzed. RESULTS: The expression in CYP2D2 and CYP2D4 mRNA decreased dramatically both in hepatocarcinoma and paracancerous tissues(P<0.01),and mRNA of the two isoforms in hepatocarcinoma tissues was lower than in paracancerous tissues(P<0.01). Though CYP2D1 mRNA had a trend of decrease in hepatocarcinoma and paracancerous tissues, no significant difference was found between them. Sequence analysis of those PCR products showed that only a single-site base mutation happened in CYP2D2 from hepatocarcinoma tissues. CONCLUSION: the expression of three isoforms(CYP2D1,CYP2D2 and CYP2D4) mRNA were reduced differently by oza dye DAB, DAB could result in CYP2D2 gene mutation, these indicate that the variants of CYP2D genes may contribute to the progression of hepatocarcinoma. 　　

Key words: dimethylaminoazobenzene, hepatocarcinoma, CYP2D1 gene, CYP2D2 gene, CYP2D4 gene, mutation