关键词: FHIT基因, p16基因, 甲基化, 肺癌, 甲基化特异性聚合酶链技术

Abstract: BACKGROUND AND AIM: To illustrate the relationship between the methylation of FHIT and p16 genes and the development of lung cancer. MATERIALS AND METHODS: Methylation of the promoters of FHIT gene and p16 gene was evaluated by methylation-specific PCR in 59 lung cancer tissues; 32 adjacent non-carcinoma tissues and 11 bronchal epithelial squamous tissues. RESULTS: FHIT methylation in lung cancer tissue samples; adjacent non-carcinoma tissue samples were 37.3%(22/59) and 0%(0/32)respectively; with significant difference(P<0.01). Methylation was found in 2 of 11 (18.1%) bronchal epithelial squamous samples. p16 methylation of lung cancer tissue samples; adjacent non-carcinoma tissue samples were 50.8%(30/59)and 0.0%(0/32)respectively; showing a significant difference(P<0.01); Methylation was found in 2(18.1%)of 11 bronchal epithelial squamous samples. FHIT/p16 combined detection of methylation found many more positive tissues in smoking patients than the single gene. There was a significant difference between smokers and non-smokers(P<0.05). CONCLUSION: The 5＇- CpG island methylation of FHIT and p16 was frequent in lung cancer and may be an early event in lung carcinogenesis.

Key words: FHIT gene, p16 gene, methylation, lung cancer, methylation-specific PCR