癌变·畸变·突变 ›› 2007, Vol. 19 ›› Issue (1): 19-021.doi: 10.3969/j.issn.1004-616x.2007.01.006

• 论著 • 上一篇    下一篇

结直肠癌、息肉及癌旁黏膜中二胺氧化酶表达的研究

汤　菲1；单保恩2,；陈　兴1；张　文1   

  1. 1.解放军白求恩国际和平医院，河北　石家庄 050082；2.河北医科大学第四医院，河北　石家庄 050011
  • 收稿日期:2005-11-29 修回日期:2006-05-22 出版日期:2007-01-30 发布日期:2007-01-30

Expression of Diamine Oxidase in Colorectal Carcinomas, Polyps and Mucosa Adjacent to Colorectal Cancer

TANG Fei1, SHAN Bao-en2, CHEN Xin1, ZHANG Wen1   

  1. 1.Bethune International Peace Hospital of PLA, shijiazhuang, Hebei 050082,China;2. NO.4 Hospital Attached to the Hebei Medical University,Shijiazhuang 050011,Hebei China
  • Received:2005-11-29 Revised:2006-05-22 Online:2007-01-30 Published:2007-01-30

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摘要： 背景与目的： 制备抗人二胺氧化酶(DAO)单克隆抗体，分析结直肠组织中DAO的表达对结直肠肿瘤辅助诊断的价值和与临床病理参数之间的关系。材料与方法： 应用免疫组织化学法检测不同结直肠病变组织中DAO的表达情况，比较其表达与结直肠肿瘤临床病理特征的关系。 结果： DAO阳性定位于细胞浆。DAO在结直肠癌、腺瘤性息肉和癌旁黏膜组织中的表达率分别为80.0％、45.7％及3.39％，差别显著，并且在绒毛状腺瘤中的表达又显著高于管状腺瘤(P<0.05)。DAO在不典型增生结直肠组织中表达阳性，并随增生程度而递增。结论： DAO可能与结直肠肿瘤发生有关，是结直肠癌发生的早期异常生物标记，能较早预测肿瘤的发生。

关键词: 二胺氧化酶, 结直肠肿瘤, 免疫组织化学

Abstract: BACKGROUND ＆ AIM: We made anti-human diamine oxidase (DAO) monoclonal antibody, analyzed the expression of diamine oxidase in colorectal tissue to assess its significance in pathologic diagnosis and its correlation with clinicopathologic parameters. MATERIALS AND METHODS: We used immunohistochemical technique to study the expressions of DAO in different colorectal tissues.The expression was compared with clinicopathological features of colorectal neoplasia. RESULTS: DAO positive expression was found in cell cytoplasm. There were significant differences among the colorectal carcinomas, tous adenomatous polyps and mucosa adjacent to cancer. DAO positive expression rate in villous adenoma was significantly higher than that of tubulo adenoma(P<0.05). DAO was expressed in dysplatic tissues, and frequency increased with the progression of the dysplasia. CONCLUSION: DAO may be associated with the growth of colorectal neoplasia. It could be an early biomarker in human colorectal carcinomas and could predict growth of tumor at an early stage.

Key words: diamine oxidase, colorectal neoplasia, immunhistochemistry

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