三氧化二砷对肺癌移植瘤的放疗增敏效应

doi:10.3969/j.issn.1004-616x.2011.01.006

›› 2011, Vol. 23 ›› Issue (1): 22-025.doi: 10.3969/j.issn.1004-616x.2011.01.006

三氧化二砷对肺癌移植瘤的放疗增敏效应

任庆兰1,吴永忠2,陈晓品1,李少林3,金丹1

1. 重庆医科大学附属第一医院肿瘤科，重庆 400016； 2. 重庆市肿瘤医院放疗科，重庆 400030；3. 重庆医科大学基础医学院放射医学教研室，重庆

收稿日期:2010-06-18 修回日期:2010-10-09 出版日期:2011-01-30 发布日期:2011-01-30
通讯作者: 任庆兰

Radiosensitization effects of arsenic trioxide in lung cancer xenografts

REN Qing-lan1,WU Yong-zhong2, CHEN Xiao-pin1, LI hao-lin3, JIN Dan1

1.Department of Oncology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 2. Department of Radiotherapy, The Chongqing Cancer Hospital, Chongqing 3. Department of Radiology, School of Basic Medical Science; Chongqing Medical University, Chongqing

Received:2010-06-18 Revised:2010-10-09 Online:2011-01-30 Published:2011-01-30

摘要/Abstract

摘要： 探讨三氧化二砷(As2O3)对肺癌移植瘤的放疗增敏作用及其可能机制。方法：在C57BL/6小鼠右前肢腋窝皮下接种Lewis肺癌细胞，建立肿瘤小鼠模型。将40只荷瘤小鼠随机分成4组：对照组(腹腔注射生理盐水0.2 ml)；单纯照射组(腹腔注射生理盐水0.2 ml，6 MeV电子线照射,10 Gy)；As2O3组(2.0 mg/kg，溶于0.2 ml生理盐水中，腹腔注射)；As2O3＋照射组(2.0 mg/kg 腹腔注射As2O3后，6 MeV电子线照射,10 Gy)。实验结束后，处死各组动物，分离肿瘤，称取瘤质量，计算抑瘤率。用RT-PCR法测定肿瘤组织中VEGF和Ku70 mRNA水平，免疫组织化学法测定肿瘤组织中VEGF和Ku70蛋白的表达。结果：成功建立Lewis肺癌荷瘤小鼠模型。与对照组、As2O3组及单纯照射组比较，As2O3＋照射组的抑瘤率明显增高，而VEGF和Ku70 mRNA和蛋白表达均显著下调(P均<0.05)。结论： As2O3能增加肺癌移植瘤的放射敏感性，其辐射增敏作用可能与下调照射后肿瘤组织VEGF和Ku70的表达有关。

关键词: 三氧化二砷, 肺癌, 放射敏感性

Abstract: To explore the effects of arsenic trioxide on radiosensitivity and the possible mechanism in lung cancer xenografts. METHODS: The tumor-bearing C57BL/6 mouse model was established by injecting Lewis lung cancer cells into right infra-axillary dermis. 40 mice were randomized into 4 groups: control group(peritoneal injection 0.9％NS,0.2 ml); As2O3 group(peritoneal injection,2.0 mg/kg,0.2 ml As2O3); irradiation group(peritoneal injection 0.9％NS,0.2 ml＋6MeV Electron Beam,10 Gy); As2O3＋irradiation group(peritoneal injection, 2.0 mg/kg, 0.2 ml As2O3＋6MeV Electron Beam,10 Gy). After experiments, mice were sacrificed, then the tumors were separated and weighed. Response to irradiation was evaluated by tumor- inhibiting rate. The expressions of VEGF and Ku70 mRNA were evaluated by RT-PCR. The protein levels of VEGF and Ku70 were measured by immunohistochemical staining. RESULTS: The Lewis lung cancer-bearing model was successfully established in mice. As2O3＋irradiation could significantly increase the tumor-inhibiting rate and downregulate the expression of VEGF and Ku70 of irradiated carcinoma xenografts, (Compared with As2O3 group or irradiation group, P＜0.05). CONCLUSION: Arsenic trioxide could sensitize lung cancer xenografts to ionizing irradiation. This effect was probably mediated by downregulating the expressions of VEGF and Ku70.

Key words: arsenic trioxide, lung cancer, radiosensitivity

任庆兰1,吴永忠2,陈晓品1,李少林3,金丹1. 三氧化二砷对肺癌移植瘤的放疗增敏效应[J]. , 2011, 23(1): 22-025.

REN Qing-lan1,WU Yong-zhong2, CHEN Xiao-pin1, LI hao-lin3, JIN Dan1. Radiosensitization effects of arsenic trioxide in lung cancer xenografts[J]. , 2011, 23(1): 22-025.

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三氧化二砷对肺癌移植瘤的放疗增敏效应

Radiosensitization effects of arsenic trioxide in lung cancer xenografts

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