Abstract: V79 Chinese hamster cells were treated in vit ro by at ropine sulfate , which is an antagonist to cholinergic receptors and inhibitor of muscarinic receptor. The alteration of late telophase2early G1 configuration induced by at ropine were determined by analyzing present or lack of a cytokinesis furrow in late telophase and very early G1 cells , The abnormal cytokinesis were investigated for the f requency of binuclear cells in interphase cells.The tested chemical disturbed mitotic coordination and induced significantly high f requency of binuclear cells.The result indicated that at ropine is able to inhibit mammal mitosis by blocking the function of cholinergic receptor. The malfunction of cholinergic receptors may be a mechanism of aneuploid induction.