基因重组人碱性成纤维细胞生长因子( rh-bFGF) 致突变研究

doi:10.3969/j.issn.1004-616x.1999.04.010

Carcinogenesis, Teratogenesis & Mutagenesis ›› 1999, Vol. 11 ›› Issue (4): 191-194.doi: 10.3969/j.issn.1004-616x.1999.04.010

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STUDY ON MUTAGENICITY ON RECOMBINANT HUMAN BASIC FIBROBLAST CROWTH FACTOR

Ma Mingfu1 , Li Lianbing1 , Cai Min1 , Li Xinsheng1 , Zeng Weisan1 , Liao Mingyang2 , Wang Zhiqiao2

1Chongqing Family Planning Scientif ic Research Instit ute , Chongqing 　400020 　2 Instit ute of Pharmacology and Toxicology , Academy of Military Medical Sciences , Beijing 　100850

Received:1998-10-28 Revised:1999-03-01 Online:1999-07-30 Published:1999-07-30

Abstract

Abstract: This paper reported the research result s of mutagenicity of rh2bFGF. The result s showed that rh -FGF at 5 doses of 0. 1 - 500μg/ plate did not induce positive mutation of st rains TA97 、TA98 、TA100 and TA10 with or without S9mix in the Ames Test . The rates of CHL Chromosome aberrations of rh - bFGF at 4 doses were negative range ( < 5 %) . The rates of micronuleus assay of polychromatic erythrocytes in mice of rh -bFGF at doses of 0. 53μg/ KG·bw ,2. 13μg/ KG·bw , 85μg/ KG·bw ,34μg/ KG·bw would not be to induce increase of it s micronuclei. It is proved that rh - bFGF would not be any mutagenicity in this experiment .

Key words: rh - bFGF, Ames test, Chromosome abcrration, Micronucleus, Mutation

Ma Mingfu , Li Lianbing , Cai Min , Li Xinsheng , Zeng Weisan , Liao Mingyang , Wang Zhiqiao. STUDY ON MUTAGENICITY ON RECOMBINANT HUMAN BASIC FIBROBLAST CROWTH FACTOR[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1999, 11(4): 191-194.

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STUDY ON MUTAGENICITY ON RECOMBINANT HUMAN BASIC FIBROBLAST CROWTH FACTOR

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