Carcinogenesis, Teratogenesis & Mutagenesis ›› 1999, Vol. 11 ›› Issue (5): 219-221.doi: 10.3969/j.issn.1004-616x.1999.05.003

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EFFECT OF AGING ON THE ACTIVITY OF CYP3A IN THE SENESCENCEACCELERATED MOUSE( SAM) LIVERS

Liu Hui1 , Chen J un Pao1 , Kei Ichi Hirai2   

  1. 1Department of Neurology , Zhujiang Hospital , Guangz hou 　510282 , 2Department of A natomy , Kanaz aw a medical University , Kanazawa , Japan
  • Received:1999-03-17 Revised:1999-05-10 Online:1999-09-30 Published:1999-09-30

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Abstract: Aging2related changes have been evaluated in hepatic cytochrome P450 content in rat s by specific cytochrome P450 enzymes. To determine whether senescence is concerned with CYP3A activity , the activities of the SAM hepatic cytochrome P450 3A (CYP3A) were quantified in vit ro as erythromycin N2demethylation in microsomes prepared f rom SAM2R1 、SAM2P1 and SAM2P8 , espectively , at 7 、13 and 36 weeks of age in every animal group. We found CYP3A activity was decreased with advancing age in SAM2P1 and SAM2P8 . At 13weeks of age , CYP3A activity was about 39. 5 % lower ( t = 2. 525 , P < 0. 05) in SAM2P1 and about 43. 7 % lower ( t = 2. 24 , P < 0. 05) in SAM2P8 . Compared with 36 to 13 weeks of age these two groups , CYP3A activity was decreased approximately 71. 3 % ( t = 2. 84 , P < 0. 02) in SAM2P1 and 62. 9 % ( t = 3. 21 , P < 0. 01)in SAM2P8 . It w as no signif icant dif f erences f rom 7 to 13 weeks of age in SAM2R1 , but f rom 13 to 36 weeks of age , it w as decreased about 38. 2 % ( t = 2. 37 , P < 0. 05) ) . Taken together , the data suggest that CYP3A takes very important effect to senescence.

Key words: Cytochrome P450 3A(CYP3A), Senescence2accelerated mouse (SAM), Senescence