人乳头瘤病毒16 E6 与食管癌细胞核基质的关系

doi:10.3969/j.issn.1004-616x.2002.02.001

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2002, Vol. 14 ›› Issue (2): 67-070.doi: 10.3969/j.issn.1004-616x.2002.02.001

THE RELATION BETWEEN HUMAN PAPILLOMAVIRUS 16 E6 AND NUCLEAR MATRIX OF

CHEN Hai-bin12; CHEN Ling1; ZHANG Jin-kun1; SHEN Zhong-ying1; SU Zhong-jing1; CHENG SB3;CHEW EC4

1. Tumor Pathology Laboratory; 2. Department of Histology and Embryology, Shantou University Medical College, Shantou 515031, China; 3. Department of Physiology; 4. Department of Anatomy, The Chinese University of Hong Kong, Shatin, NT. Hong Kong, China

Received:2001-09-29 Revised:2001-12-03 Online:2002-04-30 Published:2002-04-30
Contact: CHEN Hai-bin

Abstract

Abstract: Purpose : To explore the etiologic role of HPV infection in sophageal carcinoma , the association of HPV-16 E6 with the nuclear mat rix of carcinoma cells was investigated. Methods : Two esophageal carcinoma cell lines , EC/ CUHK1 and EC/ CUHK2 , were tested for HPV-16 E6 subgenic fragment by using polymerase chain reaction amplification of virus DNA associated nuclear matrix. RT-PCR and immunocytochemist ry were used to visualize the expression of E6 subgene in the cells. Results : The HPV-16 E6 subgenic fragment were found to be present in nuclear mat rix-associated DNA , E6 oncoprotein localized in the nucleus where it tightly associate with nuclear mat rix after sequential extraction in EC/ CUHK2 cells. This phenomenon was not detected , however , in EC/ CUHK1 cells. Conclusion : The interaction between HPV-16 E6 and nuclear mat rix may cont ribute to the viral induced carcinogenesis in esophageal carcinoma cell line EC/ CUHK2.

Key words: esophageal carcinoma, nuclear matrix, human papillomavirus

CLC Number:

CHEN Hai-bin, CHEN Ling, ZHANG Jin-kun, SHEN Zhong-ying, SU Zhong-jing, CHENG SB, CHEW EC. THE RELATION BETWEEN HUMAN PAPILLOMAVIRUS 16 E6 AND NUCLEAR MATRIX OF[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2002, 14(2): 67-070.

[1]	CAI Xiaoman, CHEN Suzuan, YU Jing, LIU Zhaohui, WU Ruinuan, TANG Shengxin, GUO Guanghua. The significance and expression of caveolin-1 and E-cadherin in esophageal carcinoma cell line TE1 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(3): 227-230.
[2]	GAO Zhikui, LIU Ran, YIN Lihong, PU Yuepu. A case-control study on the risk of esophageal carcinoma and miR-338 cluster [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(2): 107-110.
[3]	CHEN Haijun, LIU Yuqin, LI Yuze, WANG Xin, LI Nan, LI Dianzhong. Vitamin E succinate inhibits proliferation and induces apoptosis in human esophageal carcinoma cells TE-1 and Eca-109 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 357-360.
[4]	SHI Wen-rong，LIU Yan*. Effects of methyl benzyl nitrosamine on β-catenin and p-β-catenin(Ser675) in esophageal precancerous lesions in Wistar rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2014, 26(5): 334-338.
[5]	GAO Zhi-di，SHAO Wei，LI Li，MA Xiao-ye， SUN Yu-ping. The correlation between HPV infection subtype and lung squamous cell carcinoma in Qingdao region [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(2): 111-114.
[6]	LIU Ling，LI Hui，WANG Hong-jiang，PANG Zuo-liang，ZHANG Rong-xiu，LI Hui-wu. The clinical significance and methylation state of survivin promoter CpG island in Kazak's esophageal cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(1): 39-43.
[7]	JING Shao-wu1;WANG Ya-di1; ZHENG Ming-min1;SUN Guo-gui; LIU Qing;CHENG Yun-jie;YANG Cong-rong;LI Cheng-lin. Effect of HIF-1α by RNAi on radiosensitivity in esophageal squamous carcinoma cell line Eca109 under hypoxia [J]. , 2012, 24(5): 325-329.
[8]	TANG Yong-fei;ZHAN Na;HUANG Ya-bing;LIU Lin. Clinicopathological implication of HER-2/neu protein expression and gene amplication in esophageal carcinoma [J]. , 2012, 24(4): 256-260.
[9]	SUN Guo-gui，HU Wan-ning，WANG Ya-di，LI Yun-ming，JING Shao-wu，LI Cheng-lin，CUI Da-wei. Relationship between characteristics of MnSOD expression and radiosensitivity [J]. , 2012, 24(2): 100-107.
[10]	LIU Xiao-xia，HAO Yu-shuan，LI Ju-mei，LIU Dong-qing，CHEN Jian-hua，YAO Yan-bing. Effects of Baohuoside-I and 5-FU on the proliferation and apoptosis of human esophageal carcinoma cell Eca-109 [J]. , 2011, 23(5): 367-369.
[11]	YANG Hai-bo，CHEN Lan-ying*，SUN Jie，XIE Zhao-hui. The effect of retinoic acid on expression of nuclear matrix protein in human esophageal cancer cells [J]. , 2011, 23(5): 377-379.
[12]	SU Zhong-jing, CHEN Hai-bin, SONG Xu-hong, XU Xiao-yuan, WANG Ning, LIU Xiao-hui, HUANG Dong-yang, . The effects of all_trans retinoic acid on the cytoskeleton and growth of esophageal carcinoma cell line EC109 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(6): 428-432.
[13]	LI Qi-feng, WU Ming-yao. The expression of EGFR, VEGFR1/Flt_1 in esophageal squamous cell carcinoma tissues with or without radiotherapy [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(5): 386-390.
[14]	. [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(2): 86-090.
[15]	GAO Shu-ying, LI En-min, DU Ze-peng, LU Xiao-feng, XU Li-yan, . TPA Responsiveness of ezrin Gene Promoter in Esophageal Carcinoma Cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2009, 21(5): 358-361.

THE RELATION BETWEEN HUMAN PAPILLOMAVIRUS 16 E6 AND NUCLEAR MATRIX OF

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments