Carcinogenesis, Teratogenesis & Mutagenesis ›› 2003, Vol. 15 ›› Issue (3): 141-143.doi: 10.3969/j.issn.1004-616x.2003.03.004

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DNA DAMAGE INDUCED BY BENZO[a]PYRENE WAS MAGNIFIED AFTER PRE-TREATMENT OF AROCLOR1254 IN Hep G2 CELLS

Wu Xin-Jiang 1, Lu Wen-Qing 1, Mersch-Sundermann V 2   

  1. 1. Departement of Occupational and Environmental Health, Tongji Medical College, Huazhong University of Science and Techlology, Wuhan 430030, China; 2. Department of Indoor and Environmental Toxicology, Faculty of Medicine, University of Giessen Giessen 35385, Germany
  • Received:2002-10-18 Revised:2003-01-04 Online:2003-07-30 Published:2003-07-30
  • Contact: Lu Wen-Qing

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Abstract: Purpose: To investigate the change of magnitude of DNA damage induced by B [a]P(benzo [a]pyrene) in Hep G2 cells after pre-treatment with Aroclor1254. Methods: single-cell gel electrophoresis(SCGE) was performed to assay DNA damages caused by aroclor1254(23, 46, 92 and 184 μmol / L) for 24 h alone treatment and B[a] P for 1 h treatment after 24 h pretreatment with Aroclor1254 in Hep G2 cells.Results:DNA damagesinduced by B[a] P increased 8 ％, 16 ％ and 160 ％ respectively in a dose-response manner after pretreatment of Aroclor1254 at 46, 92 and 184 μmol / L compared to the B[a]P treatment alone. A statistically significant increase of DNA damage caused by B[a]P was observed at the concentration of 184 μmol / L of Aroclor1254 pretreatment(P<0.01). Conclusion: DNA damage induced by B[a]P can be enhanced by Aroclor1254, which may be a result from induction of CYP1A1 by Aroclor1254.

Key words: aroclor1254, benzo[a]pyrene, single-cell gel electrophoresis(SCGE)