硝酸羟胺诱导小鼠淋巴瘤细胞tk基因突变的研究

doi:10.3969/j.issn.1004-616x.2004.06.015

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2004, Vol. 16 ›› Issue (6): 365-367.doi: 10.3969/j.issn.1004-616x.2004.06.015

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Studies of tk Gene Mutation by Hydroxylammonium Nitrate

JIA Qing-jun, LIU Sheng-xue, LIU Jin-yi, et al

Department of Hygienic Toxicology,Preventive Medicine College,Third Millitary Medical University,Chongqing 400038,China

Received:2004-06-15 Revised:2004-08-10 Online:2004-11-30 Published:2004-11-30
Contact: CAO Jia

Abstract

Abstract: BACKGROUND ＆ AIM: To investigate the effects of Hydroxylammonium Nitrate (HAN) on the tk gene of mouse lymphoma cells. MATERIAL AND METHODS: L5178Y cells were treated with Hydroxylammonium Nitrate with different concentrations, to determine cytotxicity and mutation at different time phases. The numbers of positive wells were counted . The cell plating efficiency, relative suspension growth and mutation frequency were determined. RESULTS: The results showed that HAN (50～500 μg/ml) induced tk gene mutation with mutation frequency 1～19 times higher than that of spontaneous mutation frequency of L5178Y cells and severe cytotoxic responses. CONCLUSION: Hydroxylammonium Nitrate can exert cytotocicity and mutagenic effects on tk geng in L5178Y cells.

Key words: hydroxylammonium nitrate(HAN), tk gene, mutagenesis, L5178Y cell

JIA Qing-jun, LIU Sheng-xue, LIU Jin-yi, et al. Studies of tk Gene Mutation by Hydroxylammonium Nitrate[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2004, 16(6): 365-367.

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Studies of tk Gene Mutation by Hydroxylammonium Nitrate

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