Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (2): 116-118.doi: 10.3969/j.issn.1004-616x.2006.02.010

Previous Articles     Next Articles

Effects of Lanthanide Ions in Rare Earths on Genomic DNA of E.coli

WANG Cheng-run1; CHEN Hua-bo1; Yang Fan2; GUO Han-qing2; WANG Zi-yao2; DING Tie-lin2   

  1. 1.Department of Chemistry and Biology, Huainan Normal College, Huainan 232001,Anhui,China; 2. Undergraduates of Year 2002, Department of Chemistry and Biology, Huainan Normal College, Huainan 232001,Anhui,China
  • Received:2005-07-04 Revised:2005-09-20 Online:2006-03-30 Published:2006-03-30
  • Contact: WANG Cheng-run

PDF (PC)

2551

Abstract: BACKGROUND ＆ AIM: To investigate genotoxic effect of lanthanide ions on prokaryote by observing the effects of lanthanide nitrate on genomic DNA of E.coli. MATERIALS AND METHODS: TreatmentⅠ: E.coli was cultured in LB medium with different concentrations of lanthanide nitrate (10,40,80,160,320 mg/L), LB medium without lanthanide nitrate was the control. Genomic DNA was extracted at 10, 15, and 20 h. Then Agarose gel electrophoresis was performed Treat mentⅡ: E.coli was cultwred in LB medium with different concentrations of lanthanide nitrate (50,200,400,800,1 600 mg/L) LB medium without lanthanide nitrate was the control. Genomic DNA was extracted at 30 h. Then Agarose gel electrophoresis was performed RESULTS: DNA degradation or cross-linkage was produced by high concentrations of lanthanide ions. CONCLUSION: Lanthanide ions affected degradation and crosslinking on genomic DNA of E.coli.

Key words: rare earths, lanthanide ions, E.coli, genomic DNA, DNA degradation, DNA crosslink

CLC Number: