Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (4): 310-313.doi: 10.3969/j.issn.1004-616x.2006.04.015

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Screening NF-kappa B Element Binding Protein of Esophageal Carcinoma Cell Line SHEEC With Yeast One Hybrid System

NIU Yong-dong1, XU Li-yan2，HAN Min1,3, FANG Wang-kai1, SHEN Zhong-ying2, LI En-min1   

  1. 1.Biochemistry and Molecular Boilogy Department, Medical College of Shantou University;2.Department of Tumor Pathology;3.Department of Emergence Surgery,the Second Hospital of Shantou Medical College,Shantou 515041, Guangdong,China
  • Received:2006-04-04 Revised:2006-05-22 Online:2006-07-30 Published:2006-07-30
  • Contact: LI En-min

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Abstract: BACKGROUND＆AIM： This study was to screen an esophageal cancer cell line (SHEEC) cDNA library for the cDNA that expressed a protein that can specifically bind the NF-kappa B element, for further understanding the expression and regulation mechanism of the NGAL(neutrophil gelatinase-associated lipocalin) gene, and the course for the over-expression of the NGAL gene in the esophageal epithelial squamous carcinoma. MATERAL AND METHODS：A tandem three oligo-nucleotides of NF-kappa B was employed as a bait in a yeast one hybrid system to screen the cDNA library of the human esophageal carcinoma cell line (SHEEC). The cDNA clones obtained were sequenced and analyzed with bioinformatics tools. RESULTS： Among the thirty clones obtained, four types of clones could encode proteins, two clones were hypothetical proteins, two types of clones were mitochondrial genes, and three types of clones were non-encoding sequences. CONCLUSION： Using the yeast one hybrid system to screen the cDNA library for the NF-kappa B core element is a preliminary work that needs confirmation by further study.

Key words: yeast one hybrid, esophageal carcinoma, cDNA libriary

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