Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (1): 26-028.doi: 10.3969/j.issn.1004-616x.2007.01.008

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Effects of Hyaluronan in Proliferation, Adhesion and Invasion Abilities of Human Colon Carcinoma Cell SW480 in Vitro

ZHUO Wen-lei1,WANG Yan2,CHEN Zheng-tang1,   

  1. 1.Cancer Research Center of Xinqiao Hospital, Third Military Medical University, Chongqing 400037;2.Institute of Respiratory Disease research，Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
  • Received:2005-07-05 Revised:2005-12-29 Online:2007-01-30 Published:2007-01-30

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Abstract: BACKGROUND ＆ AIM: Hyaluronan(HA), a kind of polysaccharide with high molecular weight, is present extensively in the mesenchyme of colon carcinoma. The objective of this study was to investigate the effects of HA on proliferation, adhesion and invasion abilities of human colon carcinoma cell SW480 in vitro. MATERIALS AND METHODS: cultured SW480 cells were divided into three groups: control group (C): cultured with serum-free medium(SFM); HA1 and HA2 treatment groups: cultured with SFM containing different concentrations of HA (HA1: 10 μg/ml, HA2: 20 μg/ml). After some time, proliferation ability was examined by MTT assay and soft agar clone formation assay, while adhesive and invasive abilities were assessed by plate adhesion model and Boyden chamber transwell assay. RESULTS: Compared with that in C group，in HA1 and HA2 treatment groups, the number of proliferative cells，soft agar cell clone formation, cell adhesion on plate and septum permeation of Boyden chamber increased significantly in HA1 and HA2 treatment groups in a HA dose-dependent manner(P<0.05). CONCLUSION: HA could enhance the proliferation, adhesion and invasion abilities of human colon carcinoma cell SW480 in vitro.

Key words: hyaluronan, proliferation, adhesion, invasion, SW480 cell line

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