Abstract: BACKGROUND ＆ AIM: To prepare green fluorescent protein(GFP) polyclonal antibody. MATERIALS AND METHODS: Muscle from one GFP-transgenic mouse was taken as antigen, mixed with adjuvant sufficiently,then immunized homogeneous mice. Blood was collected 4 weeks later and the serum was used for immunocytochemistry, immunohistochemistry and western blot. RESULTS: The serum we collected showed strong positive response when diluted at 1∶200. CONCLUSION: Green fluorescent protein polyclonal antibody could be prepared directly with muscle of GFP-transgenic mouse, with which we efficiently detected GFP positive cells transplanted in the liver.

Key words: green fluorescent protein, polyclonal antibody, transgenic mouse