Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (2): 133-135.doi: 10.3969/j.issn.1004-616x.2007.02.015

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Expression of Heme Oxygenase-1 in Renal Cortex of Rats Acutely Poisoned by Cadmium

WEI Yan-hong, LI Long, CAO Yu-guang, SHI Nian, DAI Zhong-hua   

  1. Department of Health Toxicology and MOE Key Lab of Environment and Health, School of Public Health , Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China
  • Received:2005-11-05 Revised:2006-02-23 Online:2007-03-30 Published:2007-03-30
  • Contact: LI Long

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Abstract: BACKGROUND ＆ AIM: To observe the changes of heme oxygenase-1 (HO-1) expressed in renal cortex of rats with acute cadmium exposure. MATERIALS AND METHODS: 24 male SD rats were divided randomly into 4 groups. There were a control group and the three experimental groups received daily intraperitoneal injections of CdCl2 (0.4,0.8,1.6 mg/kg) for five days. 0.9％ NaCl substituted for CdCl2 in control. The specimens of kidney were prepared to measure the expression of HO-1 by RT-PCR and immunohistochemistry technique on the fifth day after injection. RESULTS: The physical expression of HO-1 was detected in control group. The levels of expressed mRNA in 0.8 and 1.6 mg/kg groups increased dramatically (P<0.05) compared with the control while the mRNA concentration of 0.4 mg/kg group was equal to the control. A dose-effect relationship between the cadmium exposure and expression of HO-1 mRNA(r=0.97,P<0.05)was also found. Analysis of immunohistochemistry showed that changes of protein expression was more sensitive than mRNA in all three groups compared with the control(P<0.05). CONCLUSION: The acute exposure to cadmium could induce HO-1 expression in renal cortex of rats and this might provide potential protection.

Key words: cadmium, kidney, mRNA, immunohistochemistry

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