基因免疫制备人PAK4单克隆抗体及其部分特性的鉴定

doi:10.3969/j.issn.1004-616x.2007.05.001

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (5): 347-350.doi: 10.3969/j.issn.1004-616x.2007.05.001

Preparation and Partial Charac_terization of the Monoclonal Antibody Specific to PAK4

HU Yuan_qing1,2, TU Chang_chun2, WEI Guang_sen3, ZHANG Hong_quan4

(1.Animal Science College, Anhui Science and Technology University,Fengyang 233100，Anhui; 2.Military Veterinary Research Institute, Academy of Military Medicine, Changchun 130062, Jilin; 3.Lanzhou Veterinary Research Institute ,Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu;4.Karolinska Institute, Department of Microbiology, Pathology and Immunology, SE_141 86 Huddinge,Sweden)

Received:2007-03-29 Revised:2007-05-23 Online:2007-09-30 Published:2007-09-30
Contact: TU Chang_chun

Abstract

Abstract: BACKGROUND ＆ AIM: For clinical diagnosis of malignant tumors and research the function of PAK4 protein, we prepared the McAbs of PAK4 protein. MATERIALS AND METHODS: The Balb/C mice were immunized with the recombinant plasmid of PAK4(the vector is P3XFLAG_CMV_10).By cell fusion and cell cloning, the McAbs of PAK4 were prepared. RESULTS: Two strains of hybridoma cells, producing anti_PAK4 antibody, named 2D1(PAK4) a subclass of IgG and 3H4(PAK4) a subclass of IgM, were obtained after fusion followed by three or four screening. The ascites of McAbs were prepared by injecting the hybridoma cells into the mice abdomen. Indirect ELISA results showed that the ascites of McAbs revealed high affinity with the PAK4 protein at the titer of 1∶1.0×104. Immunohistochemistry showed that the McAbs of PAK4 reacted specifically with the cells of breast cancer. CONCLUSION:These results demonstrated that the McAbs of PAK4 could be a specific marker for diagnosis of cancer.

Key words: gene immunology, monoclonal antibody, PAK4, immunohistochemistry

CLC Number:

R730.45

HU Yuan_qing, TU Chang_chun, WEI Guang_sen, ZHANG Hong_quan. Preparation and Partial Charac_terization of the Monoclonal Antibody Specific to PAK4[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2007, 19(5): 347-350.

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Preparation and Partial Charac_terization of the Monoclonal Antibody Specific to PAK4

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