对PTEN缺失小鼠成纤维细胞中DNA氧化损伤的研究

doi:10.3969/j.issn.1004-616x.2008.01.002

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2008, Vol. 20 ›› Issue (1): 6-010.doi: 10.3969/j.issn.1004-616x.2008.01.002

Previous Articles Next Articles

PTEN Deletion Leads to Increased Oxidative DNA Damage in Mouse Embryonic Fibroblasts

GOU Qiao1,2 HUO Yan-ying1,, HU Ying-chun1, LI Gang1, WU De-chang1

1. Beijing Insititute of Radiation Medicine, Beijing 100850；2. Department of Pathology, Chongqing University of Medical Science, Chongqing 400016, China

Received:2007-11-22 Revised:2007-11-30 Online:2008-01-30 Published:2008-01-30
Contact: HUO Yan-ying

Abstract

Abstract: BACKGROUND AND AIM: To study the variation of the level of reactive oxygen species(ROS), oxidative DNA damage and antioxidative ability between Pten＋/＋MEFs and Pten－/－MEFs cells. MATERIALS AND METHODS: Fluorescence activated cell sorter(FACS), immunocytochemistry (ICC) and neutral single cell gel electrophoresis(SCGE) were employed to compare the differences of the levels of ROS , 8-hydroxy-2'-deoxyguanosine(8-OH-dG),double-strand breaks(DSBs)and antioxidative ability between Pten＋/＋MEFs and Pten－/－MEFs cells. RESULTS: In Pten－/－MEFs , the levels of ROS, 8-OH-dG and DSBs were increased. Moreover, its antioxidative ability was decreased. CONCLUSION: PTEN could regulate the level of ROS to affect oxidative DNA damage.

Key words: phosphatase and tensin homolog deleted on chromosome ten, ROS, oxidative DNA damage

GOU Qiao, HUO Yan-ying, , HU Ying-chun, LI Gang, WU De-chang. PTEN Deletion Leads to Increased Oxidative DNA Damage in Mouse Embryonic Fibroblasts[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(1): 6-010.

[1]	SUN Huixin, ZHANG Maoxiang, WANG Wanying, JIA Haihan, SONG Bingbing. Epidemiological characteristics of prostate cancer in tumor registration areas of the Heilongjiang Province from 2013 to 2017 [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 345-348.
[2]	ZHANG Shimin, ZHAO Ruijun. Expression of protein tyrosine phosphatase receptor J in breast cancer tissues and its relationship with clinical prognosis [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 276-279,285.
[3]	ZHANG Shilei, YOU Shuping, ZHAO Jun, LIU Tao. Protective-effect-of Cistanche phenylethanoid glycoside liposomes on BSA induced liver fibrosis in rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(6): 428-433.
[4]	WANG Ziyi, ZHANG Lulu, SUN Zhenxiao, LIU Changzhen. Fludarabine combined with TRAIL induced apoptosis in lung cancer A549 cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(3): 203-207.
[5]	HU Jie, CHAO Jianqian. Utilizing the TCGA datasets to show clinical significance of FoxO1 expression in prostate cancers [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(3): 198-202.
[6]	CHEN Tingyu, WANG Dongwei, ZHANG Jinbo, WANG Wei, WANG Jingtao, SHENG Yanliang, LU Chunfeng. Involvement of the ROS/Caspase-3 signaling pathway in isoniazid-induced apoptosis in L-02 cells and the protective effect of quercetin [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(1): 53-57,68.
[7]	YOU Shuping, ZHANG Shilei, ZHAO Jun, MA Long, LIU Tao. Effect of verbascoside on platelet derived growth factor-BB-mediated activities in rat hepatic stellate cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(6): 473-478.
[8]	LIU Mengmeng, LI Yanwen, ZHOU Qingbiao, ZHANG Jiaxin, WANG Lele, LIU Jiangzheng, YU Weihua, KONG Deqin, LIU Rui, HAI Chunxu, ZHANG Xiaodi. Effects of salidroside on mitochondria of lung in rats with chlorine-induced acute lung injury [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 31-36,41.
[9]	SUN Beilei, ZHENG Yuqiong, LIN Ling, CHEN Weiying, CAI Erhui, CHEN Weifang. Effects of isoimperaterin on regulation of tyrosinase and Rab27a in human epidermal melanocytes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 37-41.
[10]	LI Peng, MA Kun, WU Haoyu, WU Yanping, LI Baixiang. Mechanism of isoflavones protection of SH-SY5Y cells from atrazine [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 25-30.
[11]	WU Shuang, LI Dengke, LI Kaiming, ZHOU Ming, SUN Zhenxiao. Hepatotoxicity of aqueous extract of Polygoni Multiflori Radix with or without the rat liver microsome incubation [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(6): 450-453.
[12]	ZHAN Biao, SUN Beilei, LUO Sicong, ZHUANG Hongkai, HUANG Pengzhou, ZENG Yi, CHEN Weifang, CHEN Weiying. Regulatory effects of isoimperatorin on expression of tyrosinase and β-actin in cultured human epidermal melanocytes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5): 364-369.
[13]	MA Kun, WU Haoyu, LI Peng, WANG Li, LI Baixiang. Induction of dopaminergic neuron degeneration by atrazine in substantia nigra in adolescent rats [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(4): 251-255.
[14]	ZHANG Haiyan, LI Qingye, CHEN Shen, CHEN Wen, HE Zhini. Construction of an environmental pollution-responsive gene-specific pyrosequencing assay [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 81-86.
[15]	XU Yu, ZHANG Xiaohong, BAN Yuanyuan, FENG Qin, ZHANG Xue, CHANG Xiling, GAO Qichao. The effect of Chlorpyrifos on the growth of Drosophila melanogaster [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(6): 477-480.

PTEN Deletion Leads to Increased Oxidative DNA Damage in Mouse Embryonic Fibroblasts

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments