高剂量三氯乙烯对L-02肝细胞中Rho GDIα表达的影响

doi:10.3969/j.issn.1004-616x.2008.01.004

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2008, Vol. 20 ›› Issue (1): 15-018.doi: 10.3969/j.issn.1004-616x.2008.01.004

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Rho GDIα Expression Change in L-02 Liver Cells after Treatment with High Concentration of Trichloroethylene

XING Xiu-mei1,2，ZHANG Jian-song1,，LIU Jian-jun2, XI Ren-rong2，HUANG Hai-yan2

1.Research Department of Applied Physiology, Medical College, Hunan Normal University, Changsha 410006;2. Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, Guangdong, China

Received:2007-07-26 Revised:2007-09-11 Online:2008-01-30 Published:2008-01-30
Contact: ZHANG Jian-song, LIU Jian-jun

Abstract

Abstract: BACKGROUND AND AIM: To study the effect of trichloroethylene(TCE) on the expression change of Rho GDIα gene at both mRNA and protein levels. MATERIALS AND METHODS: Western blot and real time quantitative RT-PCR were employed to detect the protein and mRNA levels of Rho GDIα in L-02 liver cells ,which were treated with DMSO and high concentrations of TCE. β-actin was selected as internal standard. RESULTS: The protein level of Rho GDIα was significantly up-regulated while its mRNA was significantly reduced. CONCLUSION: The Rho GDIα gene expression level showed specific changes after treatment with high concentration of TCE, providing important clues to the mechanism of TCE-induced damage in humans.

Key words: trichloroethylene, Rho GDIα, western blot, real time quantitative RT-PCR

XING Xiu-mei, ZHANG Jian-song, , LIU Jian-jun, XI Ren-rong, HUANG Hai-yan. Rho GDIα Expression Change in L-02 Liver Cells after Treatment with High Concentration of Trichloroethylene[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(1): 15-018.

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Rho GDIα Expression Change in L-02 Liver Cells after Treatment with High Concentration of Trichloroethylene

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