Carcinogenesis, Teratogenesis & Mutagenesis ›› 2008, Vol. 20 ›› Issue (3): 185-188.doi: 10.3969/j.issn.1004-616x.2008.03.005

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Protein Expression of Human Spermatozoa-mediated HBs Gene and HBc Gene in Early Embryonic Cells

ZHANG Qiu-ju, HUANG Tian-hua, XIE Qing-dong ,TAN Xiao-fang, LIU Ge-fei, CHEN De-yu, ZHOU Xiao-ling   

  1. Research Center for Reproductive Medicine, Shantou University Medical College, Shantou 515041, Guangdong,China
  • Received:2008-01-29 Revised:2008-02-28 Online:2008-05-30 Published:2008-05-30
  • Contact: HUANG Tian-hua

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Abstract: BACKGROUND AND AIM: To study the protein expression of HBs and HBc genes brought into embryos via human spermatozoa. MATERIALS AND METHODS: Human spermatozoa were transfected with recombinant plasmid pIRES2-EGFP-HBV and were then fertilized with zona pellucida-free golden hamster ova. The 2-cell embryos with and without green fluorescence were collected by immunofluorescence assay and ELISA analysis, respectively. RESULTS: The immunofluorescence assay showed that no HBsAg- and HBcAg- positive signal was observed in 2-cell embryos without green fluorescence and the clear HBsAg- and HBcAg- positive signals were detected in ones with green fluorescence. ELISA analysis showed that the amount of HBsAg in a single 2-cell embryo was about 0.064 ng/ml and the positive result for HBcAg was detected. CONCLUSION: Human sperm-mediated HBs and HBc genes are able to express their proteins in early embryonic cells.

Key words: HBV gene, human spermatozoa, embryonic cells, protein expression