Abstract: BACKGROUND AND AIM: To prepare specific monoclonal antibody (mAb) against rabbit NADP(H)-dependent retinol dehydrogenase／reductase (NRDR) for further exploration of its structure and function. MATERIALS AND METHODS: The BALB/c mice were immunized with the recombinant rabbit NRDR expressed by genetic engineering. Splenocytes of immunized mice were collected and fused with the mouse myeloma cell line NS-1 cells. Hybridomas that secreted rabbit NRDR mAb were cloned with limited dilution method. Characteristics of mAb(Ig subclasses，titers and specificities) were identified and determined by indirect ELISA and Western blot. RESULTS: From over 80 positive hybridomas which secreted anti-rabbit-NRDR mAbs，three clones of hybridoma were obtained, and designated as NR1、NR2 and NR5. They were all of IgG1 subclass. The cell culture supernatant titers of NR1,NR2 and NR5 were1∶20,1∶40 and 1∶20,respectively. Ascite titers of NR1,NR2 and NR5 mAb were 1∶106, 1∶107 and 1∶106, respectively. Western blot analysis showed that mAbs had specific binding abilities with NRDR in rabbit liver and recombinant rabbit NRDR. CONCLUSION:The hybridomas secreting mAbs to rabbit NRDR were established successfully and primarily identified，which could lay the basis for further research on the biological structure and function of NRDR.

Key words: rabbit NRDR, monoclonal antibody(mAb), characterization, ratinoic acid