Abstract: BACKGROUND AND AIM: Multi-color fluorescence in situ hybridization(MFISH) was employed to evaluate the frequency of aneuploid sperm in patients with asthenospermia. MATERIALS AND METHODS:Patients were divided into 2 groups: 6 with asthenospermia and 3 normal healthy men used as control. MFISH and probes for chromosomes including X,Y and 18 were used to determine the frequency of aneuploidy in sperms. RESULTS: Analysis was performed on 30 305 sperms. The rate of hybridization was 99.98％. Nullisomy kinds included XX18, YY18, XY18, X1818, Y1818. Nullisomy frequencies were 0.133％％±0.095％,0.135％±0.231％,0.438％±0.415％,0.507％±0.973％,0.432％±0.705％,respectively, and were 0.011％±0.020％,0.006％±0.010％,0.051％±0.075％,0.023％±0.040％,0.034％±0.034％ in control group.Aneuploidy in the sperms of test group and control group were 2.02％ and 0.25％, respectively, significantly higher in the test group(P<0.01).CONCLUSION: Sperm aneuploidy can be reliably determined by sperm MFISH analysis in men with asthenospermia.

Key words: asthenospermia, multi-color fluoescence in situ hybnidization, aneuploidy