高电压脉冲电场对K562细胞凋亡的影响

doi:10.3969/j.issn.1004-616x.2008.04.010

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2008, Vol. 20 ›› Issue (4): 288-291.doi: 10.3969/j.issn.1004-616x.2008.04.010

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Investigation of Apoptosis of K562 Cells Induced by High Intensity Pulsed Electric Fields

YIN Yong_guang1, YU Qing_yu1, WU Wei2

1.College of Biological and Agriculture Engineering, Jilin University, Changchun 130022；2.College of Vocational Technology, Changchun 130033, China

Received:2007-11-23 Revised:2008-03-22 Online:2008-07-30 Published:2008-07-30

Abstract

Abstract: BACKGROUND AND AIM: A new approach to kill tumor cells in the blood through investigating apoptosis of K562 cells induced by high intensity pulsed electric fields. MATERIALS AND METHODS: Apoptosis of K562 cells was evaluated by transmission electron microscope and Annexin V_FITC and PI double labeled flow cytometry. RESULTS: There were many vacuoles and cracks in the cells, with heterochromatin margination, nuclear pycnosis and nuclear fragmentation. The high intensity pulsed electric fields caused damage in K562 cells, and the apoptotic percentage went up with the increase in frequency and intensity of PEF. CONCLUSION: K562 cell apoptosis was induced by high intensity pulsed electric fields,providing a new approach to kill tumors in the blood.

Key words: high intensity pulsed electric fields, K562 cell, apoptosis

YIN Yong_guang, YU Qing_yu, WU Wei. Investigation of Apoptosis of K562 Cells Induced by High Intensity Pulsed Electric Fields[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(4): 288-291.

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Investigation of Apoptosis of K562 Cells Induced by High Intensity Pulsed Electric Fields

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