Carcinogenesis, Teratogenesis & Mutagenesis ›› 2008, Vol. 20 ›› Issue (5): 342-345.doi: 10.3969/j.issn.1004-616x.2008.05.002

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Effects of Periplocin on Proliferation of Human Esophageal Carcinoma Cell TE-13 and Its Relation With Rb Expression

SHAN Bao_en1, ZHAO Lian_mei1, HE Lan_xin2,DI Hong_qin3, REN Feng_zhi4   

  1. 1. Research Center; 2.Institute of Immunology, The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011; 3. New Drug Research and Development Co.,Ltd. of North China Pharmaceutical Corporation, Shijiazhuang 050015; 4.The Chest Hospital of Hebei Province,Shijiazhuang 050012, China
  • Received:2008-02-24 Revised:2008-03-22 Online:2008-09-30 Published:2008-09-30

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Abstract: BACKGROUND AND AIM: Effects of periplocin from cortex periplocae (CPP) on proliferation and apoptosis of human esophageal carcinoma cell and its relation with Rb protein expression were investigated. MATERIALS AND METHODS: Esophageal cancer cell line TE_13 was co_incubated with different concentrations of CPP and cell proliferation was measured by MTT method. Cell morphology changes were examined by light microscope. Apoptosis was studied by flow cytometry. Rb expression was analyzed by immunohistochemistry method. RESULTS: CPP could inhibit the proliferation of TE_13 cells significantly in a dose and time dependent manner (P<0.01). After treatment with CPP, TE_13 cells showed morphologic changes of apoptosis. The expression of Rb was enhanced. CONCLUSION: CPP could inhibit the growth of TE_13 cells，the mechanism might be related to enhanced Rb expression.

Key words: periplocin, esophageal carcinoma, Rb, cortex periplocae