Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (2): 85-088.doi: 10.3969/j.issn.1004-616x.2009.02.002

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Inhibiting Expression of Livin Gene in SGC-7901 Cell Line by RNA Interference

QIAO Min1, XIANG Ting-xiu2, WANG Pi-long1   

  1. 1.Department of Gastroenterology,The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016; 2.Experimental Research Center, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
  • Received:2008-07-17 Revised:2008-09-12 Online:2009-03-30 Published:2009-03-30

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Abstract: BACKGROUND AND AIM: To investigate the feasibility of gastric carcinoma gene therapy by utilizing RNA interference(RNAi) to inhibit expression of Livin in vitro. MATERIALS AND METHODS: Small interference RNA(siRNA) homologous to Livin gene were designed, pTZU6＋1-siRNA-Livin vector was constructed and transfected into SGC-7901 cells to induce RNAi in vitro.The changes of tumor cell cycles were examined with flow cytometry, Livin gene expression were measured with RT-PCR and immunochemistry, tumor cell apoptosis was teseted by TUNEL in vitro. RESULTS: After transfected with pTZU6＋1-siRNA-Livin,the percentage of S phase was reduced from 42.78％ to 19.15％(P<0.05),G1/G0 phase increased from 52.68％ to 72.98％(P<0.05). The down-regulation of Livin mRNA in group transfected with pL1-siRNA was obvious compared with the control group,with marked apoptosis. CONCLUSION: RNA interference down-regulated Livin expression, inhibited gastric carcinoma SGC-7901 cell proliferation and induced apoptosis, and may become a potential method for gene therapy of gastric cancer.

Key words: RNA interference, gastric carcinoma, cell cycle, apoptosis