FITC标记无唾液酸胎球蛋白对人精子去唾液酸糖蛋白受体的定位

doi:10.3969/j.issn.1004-616x.2009.02.013

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (2): 132-134.doi: 10.3969/j.issn.1004-616x.2009.02.013

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Locating Asialoglycoprotein Receptor on Human Sperm Using FITC-labeled Asialofetuin

SUN Ping-nan1, ZHOU Xiao-ling1, HUANG Tian-hua1,XIE Qing-dong1, CHEN Hui-fang2, KANG Xiang-jing1

1.Department of Cell Biology and Medical Genetics; 2. International Institut of Infection and Immunity, Shantou University Medical College, Shantou 515041,Guang dong, China

Received:2008-09-28 Revised:2008-12-21 Online:2009-03-30 Published:2009-03-30

Abstract

Abstract: BACKGROUND AND AIM: To locate and monitor Asialoglycoprotein receptor(ASGP-R) on the sperm surface using FITC-labeled asialofetuin(FITC-ASF). MATERIALS AND METHODS: Asialofetuin was labeled with FITC to produce FITC-ASF, which was used in the investigation of binding between foreign protein and ASGP-R on the sperm surface using immunofluorescence and flow cytometry. RESULTS: FITC-ASF was able to label ASGP-R on sperm surface well and successfully applied to assess the binding of foreign protein to the receptor. CONCLUSION: The study provided a new potential and convenient method to monitor ASGP-R.

Key words: asialofetuin, FITC, human sperm, asialoglycoprotein receptor, location

SUN Ping-nan, ZHOU Xiao-ling, HUANG Tian-hua, XIE Qing-dong, CHEN Hui-fang, KANG Xiang-jing. Locating Asialoglycoprotein Receptor on Human Sperm Using FITC-labeled Asialofetuin[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2009, 21(2): 132-134.

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Locating Asialoglycoprotein Receptor on Human Sperm Using FITC-labeled Asialofetuin

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